Imperial College London
Alternate

DrMarieLoh

Faculty of Medicine, School of Public Health

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2885m.loh

 
 
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Location

 

172Praed StreetSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chong:2010:10.1002/pmic.201000127,
author = {Chong, P and Lee, H and Loh, MCS and Choong, L and Lin, Q and So, JBY and Lim, KH and Soo, RA and Yong, WP and Chan, SP and Smoot, DT and Ashktorab, H and Yeoh, KG and Lim, YP},
doi = {10.1002/pmic.201000127},
journal = {PROTEOMICS},
pages = {3210--3221},
title = {Upregulation of plasma C9 protein in gastric cancer patients},
url = {http://dx.doi.org/10.1002/pmic.201000127},
volume = {10},
year = {2010}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>Gastric cancer is one of the leading causes of cancerrelated deaths worldwide. Current biomarkers used in the clinic do not have sufficient sensitivity for gastric cancer detection. To discover new and better biomarkers, protein profiling on plasma samples from 25 normal, 15 earlystage and 21 latestage cancer was performed using an iTRAQLCMS/MS approach. The level of C9 protein was found to be significantly higher in gastric cancer compared with normal subjects. Immunoblotting data revealed a congruent trend with iTRAQ results. The discriminatory power of C9 between normal and cancer states was not due to interpatient variations and was independent from gastritis and <jats:italic>Helicobacter pylori</jats:italic> status of the patients. C9 overexpression could also be detected in a panel of gastric cancer cell lines and their conditioned media compared with normal cells, implying that higher C9 levels in plasma of cancer patients could be attributed to the presence of gastric tumor. A subsequent blind test study on a total of 119 plasma samples showed that the sensitivity of C9 could be as high as 90% at a specificity of 74%. Hence, C9 is a potentially useful biomarker for gastric cancer detection.</jats:p>
AU  - Chong,P
AU  - Lee,H
AU  - Loh,MCS
AU  - Choong,L
AU  - Lin,Q
AU  - So,JBY
AU  - Lim,KH
AU  - Soo,RA
AU  - Yong,WP
AU  - Chan,SP
AU  - Smoot,DT
AU  - Ashktorab,H
AU  - Yeoh,KG
AU  - Lim,YP
DO  - 10.1002/pmic.201000127
EP  - 3221
PY  - 2010///
SN  - 1615-9853
SP  - 3210
TI  - Upregulation of plasma C9 protein in gastric cancer patients
T2  - PROTEOMICS
UR  - http://dx.doi.org/10.1002/pmic.201000127
VL  - 10
ER  -
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