27 results found
Borges PDN, Forte AE, Vincent TL, et al., 2014, Rapid, automated imaging of mouse articular cartilage by microCT for early detection of osteoarthritis and finite element modelling of joint mechanics, Osteoarthritis and Cartilage, Vol: 22, Pages: 1419-1428, ISSN: 1063-4584
ObjectiveMouse articular cartilage (AC) is mostly assessed by histopathology and its mechanics is poorly characterised. In this study: (1) we developed non-destructive imaging for quantitative assessment of AC morphology and (2) evaluated the mechanical implications of AC structural changes.MethodsKnee joints obtained from naïve mice and from mice with osteoarthritis (OA) induced by destabilization of medial meniscus (DMM) for 4 and 12 weeks, were imaged by phosphotungstic acid (PTA) contrast enhanced micro-computed tomography (PTA-CT) and scored by conventional histopathology. Our software (Matlab) automatically segmented tibial AC, drew two regions centred on each tibial condyle and evaluated the volumes included. A finite element (FE) model of the whole mouse joint was implemented to evaluate AC mechanics.ResultsOur method achieved rapid, automated analysis of mouse AC (structural parameters in <10 h from knee dissection) and was able to localise AC loss in the central region of the medial tibial condyle. AC thickness decreased by 15% at 4 weeks and 25% at 12 weeks post DMM surgery, whereas histopathology scores were significantly increased only at 12 weeks. FE simulations estimated that AC thinning at early-stages in the DMM model (4 weeks) increases contact pressures (+39%) and Tresca stresses (+43%) in AC.ConclusionPTA-CT imaging is a fast and simple method to assess OA in murine models. Once applied more extensively to confirm its robustness, our approach will be useful for rapidly phenotyping genetically modified mice used for OA research and to improve the current understanding of mouse cartilage mechanics.
Jones GN, Moschidou D, Abdulrazzak H, et al., 2014, Potential of Human Fetal Chorionic Stem Cells for the Treatment of Osteogenesis Imperfecta, STEM CELLS AND DEVELOPMENT, Vol: 23, Pages: 262-276, ISSN: 1547-3287
Marenzana M, Vugler A, Moore A, et al., 2013, Effect of sclerostin-neutralising antibody on periarticular and systemic bone in a murine model of rheumatoid arthritis: a microCT study, ARTHRITIS RESEARCH & THERAPY, Vol: 15, ISSN: 1478-6354
Marenzana M, Arnett TR, 2013, The Key Role of the Blood Supply to Bone, Bone Research, Vol: 1, Pages: 203-215
The importance of the vascular supply for bone is well-known to orthopaedists but is still rather overlooked within the wider field of skeletal research. Blood supplies oxygen, nutrients and regulatory factors to tissues, as well as removing metabolic waste products such as carbon dioxide and acid. Bone receives up to about 10% of cardiac output, and this blood supply permits a much higher degree of cellularity, remodelling and repair than is possible in cartilage, which is avascular. The blood supply to bone is delivered to the endosteal cavity by nutrient arteries, then flows through marrow sinusoids before exiting via numerous small vessels that ramify through the cortex. The marrow cavity affords a range of vascular niches that are thought to regulate the growth and differentiation of hematopoietic and stromal cells, in part via gradients of oxygen tension. The quality of vascular supply to bone tends to decline with age and may be compromised in common pathological settings, including diabetes, anaemias, chronic airway diseases and immobility, as well as by tumours. Reductions in vascular supply are associated with bone loss. This may be due in part to the direct effects of hypoxia, which blocks osteoblast function and bone formation but causes reciprocal increases in osteoclastogenesis and bone resorption. Common regulatory factors such as parathyroid hormone or nitrates, both of which are potent vasodilators, might exert their osteogenic effects on bone via the vasculature. These observations suggest that the bone vasculature will be a fruitful area for future research.
Marenzana M, Hagen CK, Borges PDN, et al., 2012, Visualization of small lesions in rat cartilage by means of laboratory-based x-ray phase contrast imaging, PHYSICS IN MEDICINE AND BIOLOGY, Vol: 57, ISSN: 0031-9155
Marenzana M, Chenu C, Pitsillides AA, et al., 2012, Does the vasodilatory action of PTH help account for its osteogenic effects?, 39th Annual Congress of the European-Calcified-Tissue-Society (ECTS), Publisher: ELSEVIER SCIENCE INC, Pages: S160-S160, ISSN: 8756-3282
Borges P, Midha N, Marenzana M, 2012, AUTOMATED MICROSTRUCTURAL ANALYSIS OF TIBIAL SUBCHONDRAL BONE IN A SURGICAL MODEL OF MOUSE OSTEOARTHRITIS., World Congress on Osteoarthritis, Publisher: ELSEVIER SCI LTD, Pages: S224-S225, ISSN: 1063-4584
Marenzana M, Greenslade K, Eddleston A, et al., 2011, Sclerostin Antibody Treatment Enhances Bone Strength but Does Not Prevent Growth Retardation in Young Mice Treated With Dexamethasone, ARTHRITIS AND RHEUMATISM, Vol: 63, Pages: 2385-2395, ISSN: 0004-3591
Marenzana M, Eddleston A, Vugler A, et al., 2010, DIFFERENTIAL DISTRIBUTION OF A PEGYLATED FAB ' INTO INFLAMED VERSUS NORMAL TISSUE COMPARED WITH AN IGG IN ARTHRITIS AND COLITIS MODELS, 30th European Workshop for Rheumatology Research, Publisher: B M J PUBLISHING GROUP, ISSN: 0003-4967
Eddleston A, Marenzana M, Marshall D, et al., 2009, Comparison of the distribution of an IgG and a PEGylated Fab' form of an anti-TNF-a antibody in the inflamed gut of colitic mice, Conference on Advances in Inflammatory Bowel Diseases Crohns and Research Conference, Publisher: JOHN WILEY & SONS INC, Pages: S50-S50, ISSN: 1078-0998
Eddleston A, Marenzana M, Moore AR, et al., 2009, A Short Treatment With an Antibody to Sclerostin Can Inhibit Bone Loss in an Ongoing Model of Colitis, JOURNAL OF BONE AND MINERAL RESEARCH, Vol: 24, Pages: 1662-1671, ISSN: 0884-0431
Nesbitt A, Eddleston A, Marenzana M, et al., 2009, Comparison of the Distribution of an IgG and a PEGylated Fab' Form of an Anti-TNF-alpha Antibody in the Inflamed Gut of Colitic Mice, 74th Annual Scientific Meeting and Postgraduate Course of the American-College-of-Gastroenterology, Publisher: NATURE PUBLISHING GROUP, Pages: S440-S441, ISSN: 0002-9270
Eddleston A, Marshall Q, Moore A, et al., 2008, A Short Treatment with an Antibody to Sclerostin Can Inhibit Bone Loss in an Ongoing Model of Colitis., 30th Annual Meeting of the American-Society-for-Bone-and-Mineral-Research, Publisher: AMER SOC BONE & MINERAL RES, Pages: S60-S60, ISSN: 0884-0431
Rubinacci A, Marenzana M, Cavani F, et al., 2008, Ovariectomy sensitizes rat cortical bone to whole-body vibration, CALCIFIED TISSUE INTERNATIONAL, Vol: 82, Pages: 316-326, ISSN: 0171-967X
Marenzana M, Chenu C, 2008, Sympathetic nervous system and bone adaptive response to its mechanical environment., J Musculoskelet Neuronal Interact, Vol: 8, Pages: 111-120, ISSN: 1108-7161
While bone adaptive response to its mechanical environment was considered to be controlled locally by cytokines and systemic hormones, some recent work suggests that it could also be neuronally regulated. Bone is indeed very densely innervated and many experimental and clinical studies have previously shown the involvement of the nervous system in the control of bone metabolism. The demonstration that the central nervous system regulates bone mass via the sympathetic nervous system (SNS) has prompted recent studies aimed to investigate the role of the SNS in the bone mechano-adaptive response. This review will focus on this work and summarize the evidence for a contribution of the beta-adrenergic signalling in the response of bone cells to mechanical loading. The apparent conflicting results obtained in diverse experimental models of loading and unloading, at different skeletal sites, and in relation to various hormonal levels, will be discussed. While those studies do not support a major influence of the SNS on the bone mechano-adaptive response, there is nevertheless strong evidence that the SNS is part of a complex system which contributes to the metabolic regulation of bone.
Marenzana M, De Souza RL, Chenu C, 2007, Blockade of beta-adrenergic signaling does not influence the bone mechano-adaptive response in mice, BONE, Vol: 41, Pages: 206-215, ISSN: 8756-3282
Marenzana M, Kelly DJ, Prendergast PJ, et al., 2007, A collagen-based interface construct for the assessment of cell-dependent mechanical integration of tissue surfaces, CELL AND TISSUE RESEARCH, Vol: 327, Pages: 293-300, ISSN: 0302-766X
Marenzana M, de Souza RL, Chenu C, 2006, The beta-adrenergic pathway does not modulate the mechanotransduction response occurring in trabecular and cortical bone upon loading or immobilization., 28th Annual Meeting of the American-Society-for-Bone-and-Mineral-Research, Publisher: AMER SOC BONE & MINERAL RES, Pages: S48-S48, ISSN: 0884-0431
Marenzana M, Wilson-Jones N, Mudera V, et al., 2006, The origins and regulation of tissue tension: Identification of collagen tension-fixation process in vitro, EXPERIMENTAL CELL RESEARCH, Vol: 312, Pages: 423-433, ISSN: 0014-4827
Chenu C, Marenzana M, 2005, Sympathetic nervous system and bone remodeling, JOINT BONE SPINE, Vol: 72, Pages: 481-483, ISSN: 1297-319X
Marenzana M, Shipley AM, Squitiero P, et al., 2005, Bone as an ion exchange organ: Evidence for instantaneous cell-dependent calcium efflux from bone not due to resorption, BONE, Vol: 37, Pages: 545-554, ISSN: 8756-3282
Furlan F, Jorgensen N, Jemsen J, et al., 2005, Urokinase receptor is involved in bone remodeling by influencing osteoclasts resorbtion., 27th Annual Meeting of the American-Society-for-Bone-and-Mineral-Research, Publisher: AMER SOC BONE & MINERAL RES, Pages: S371-S371, ISSN: 0884-0431
Marenzana M, Colasante F, Di Rauso U, et al., 2005, Simultaneous determination of densitometric, geometric, and histomorphometric parameters of femoral neck from pQCT scans by a dedicated image analysis program. Implications for the prediction of fracture risk, 2nd Joint Meeting of the European-Calcified-Tissue-Society/International-Bone-and-Mineral-Society, Publisher: ELSEVIER SCIENCE INC, Pages: S163-S163, ISSN: 8756-3282
Furlan F, Jorgensen N, Jemsen J, et al., 2005, Involvement of the urokinase receptor in bone homeostasis, 2nd Joint Meeting of the European-Calcified-Tissue-Society/International-Bone-and-Mineral-Society, Publisher: ELSEVIER SCIENCE INC, Pages: S295-S296, ISSN: 8756-3282
Furlan F, Jorgensen NR, Jemsen J, et al., 2005, Involvement of the urokinase receptor in bone homeostasis, 10th Interenational Workshop on Molecular and Cellular Biology of Plasminogen Activation, Publisher: SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN, Pages: A22-A22, ISSN: 0340-6245
Marenzana M, Pickard D, MacRobert AJ, et al., 2002, Optical measurement of three-dimensional collagen gel constructs by elastic scattering spectroscopy, TISSUE ENGINEERING, Vol: 8, Pages: 409-418, ISSN: 2152-4947
Marenzana M, Carlevaro M, 1997, A computer-based technique for cell quantitation: ''in vitro'' chemotactic migration of endothelial cells, EUROPEAN JOURNAL OF HISTOCHEMISTRY, Vol: 41, Pages: 101-102, ISSN: 1121-760X
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