Imperial College London
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PROFESSOR MIRIAM F. MOFFATT

Faculty of Medicine, National Heart & Lung Institute

Consul for the Faculty of Medicine, Professor
 
 
 
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Contact

 

+44 (0)20 7594 2942m.moffatt

 
 
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Location

 

400Guy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cuthbertson:2021:10.1016/j.jcf.2020.05.013,
author = {Cuthbertson, L and Felton, I and James, P and Cox, MJ and Bilton, D and Schelenz, S and Loebinger, MR and Cookson, WOC and Simmonds, NJ and Moffatt, MF},
doi = {10.1016/j.jcf.2020.05.013},
journal = {Journal of Cystic Fibrosis},
pages = {295--302},
title = {The fungal airway microbiome in cystic fibrosis and non-cystic fibrosis bronchiectasis},
url = {http://dx.doi.org/10.1016/j.jcf.2020.05.013},
volume = {20},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundThe prevalence of fungal disease in cystic fibrosis (CF) and non-CF bronchiectasis is increasing and the clinical spectrum is widening. Poor sensitivity and a lack of standard diagnostic criteria renders interpretation of culture results challenging. In order to develop effective management strategies, a more accurate and comprehensive understanding of the airways fungal microbiome is required. The study aimed to use DNA sequences from sputum to assess the load and diversity of fungi in adults with CF and non-CF bronchiectasis.MethodsNext generation sequencing of the ITS2 region was used to examine fungal community composition (n = 176) by disease and underlying clinical subgroups including allergic bronchopulmonary aspergillosis, chronic necrotizing pulmonary aspergillosis, non-tuberculous mycobacteria, and fungal bronchitis. Patients with no known active fungal disease were included as disease controls.ResultsITS2 sequencing greatly increased the detection of fungi from sputum. In patients with CF fungal diversity was lower, while burden was higher than those with non-CF bronchiectasis. The most common operational taxonomic unit (OTU) in patients with CF was Candida parapsilosis (20.4%), whereas in non-CF bronchiectasis sputum Candida albicans (21.8%) was most common. CF patients with overt fungal bronchitis were dominated by Aspergillus spp., Exophiala spp., Candida parapsilosis or Scedosporium spp.ConclusionThis study provides a framework to more accurately characterize the extended spectrum of fungal airways diseases in adult suppurative lung diseases.
AU  - Cuthbertson,L
AU  - Felton,I
AU  - James,P
AU  - Cox,MJ
AU  - Bilton,D
AU  - Schelenz,S
AU  - Loebinger,MR
AU  - Cookson,WOC
AU  - Simmonds,NJ
AU  - Moffatt,MF
DO  - 10.1016/j.jcf.2020.05.013
EP  - 302
PY  - 2021///
SN  - 1569-1993
SP  - 295
TI  - The fungal airway microbiome in cystic fibrosis and non-cystic fibrosis bronchiectasis
T2  - Journal of Cystic Fibrosis
UR  - http://dx.doi.org/10.1016/j.jcf.2020.05.013
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000633886500022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S1569199320301636?via%3Dihub
UR  - http://hdl.handle.net/10044/1/80758
VL  - 20
ER  -
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