Imperial College London
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DrMasahiroOno

Faculty of Natural Sciences, Department of Life Sciences

Reader in Immunology
 
 
 
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Contact

 

m.ono

 
 
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Location

 

605Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Bending:2017:10.1101/217687,
author = {Bending, D and Martín, PP and Paduraru, A and Ducker, C and Marzaganov, E and Laviron, M and Kitano, S and Miyachi, H and Crompton, T and Ono, M},
doi = {10.1101/217687},
title = {A Timer for analyzing temporally dynamic changes in transcription during differentiation in vivo},
url = {http://dx.doi.org/10.1101/217687},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>Understanding the mechanisms of cellular differentiation is challenging because differentiation is initiated by signaling pathways that drive temporally dynamic processes, which are difficult to analyse in vivo. We establish a new Tool, Timer-of-cell-kinetics-and-activity (Tocky [toki], time in Japanese). Tocky uses the Fluorescent Timer protein, which spontaneously shifts its emission spectrum from blue-to-red, in combination with computer algorithms to reveal the dynamics of differentiation in vivo. Using a transcriptional target of T cell receptor (TCR)-signaling, we establish Nr4a3-Tocky to follow downstream effects of TCR signaling. Nr4a3-Tocky reveals the temporal sequence of events during regulatory T cell (Treg) differentiation and shows that persistent TCR signals occur during Treg generation. Remarkably, antigen-specific T cells at the site of autoimmune inflammation also show persistent TCR signaling. In addition, by generating Foxp3-Tocky, we reveal the in vivo dynamics of demethylation of the <jats:italic>Foxp3</jats:italic> gene. Thus, Tocky is a Tool for cell biologists to address previously inaccessible questions by directly revealing dynamic processes in vivo.</jats:p><jats:sec><jats:title>Summary</jats:title><jats:p>The authors establish a new Tool, Timer-of-cell-kinetics-and-activity (Tocky) revealing the temporal dynamics of cellular activation and differentiation in vivo. The tool analyses the temporal sequence of molecular processes during cellular differentiation and identifies cells that receive persistent signals in vivo.</jats:p></jats:sec>
AU  - Bending,D
AU  - Martín,PP
AU  - Paduraru,A
AU  - Ducker,C
AU  - Marzaganov,E
AU  - Laviron,M
AU  - Kitano,S
AU  - Miyachi,H
AU  - Crompton,T
AU  - Ono,M
DO  - 10.1101/217687
PY  - 2017///
TI  - A Timer for analyzing temporally dynamic changes in transcription during differentiation in vivo
UR  - http://dx.doi.org/10.1101/217687
ER  -
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