Imperial College London

ProfessorMaryRyan

Faculty of EngineeringDepartment of Materials

Vice-Dean (Research), Faculty of Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6755m.p.ryan

 
 
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Location

 

B338Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mukherjee:2014:2014/852890,
author = {Mukherjee, D and Royce, SG and Sarkar, S and Thorley, A and Schwander, S and Ryan, MP and Porter, AE and Chung, KF and Tetley, TD and Zhang, J and Georgopoulos, PG},
doi = {2014/852890},
journal = {Journal of Toxicology},
title = {Modeling in vitro cellular responses to silver nanoparticles},
url = {http://dx.doi.org/10.1155/2014/852890},
volume = {2014},
year = {2014}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Engineered nanoparticles (NPs) have been widely demonstrated to induce toxic effects to various cell types. In vitro cell exposure systems have high potential for reliable, high throughput screening of nanoparticle toxicity, allowing focusing on particular pathways while excluding unwanted effects due to other cells or tissue dosimetry. The work presented here involves a detailed biologically based computational model of cellular interactions with NPs; it utilizes measurements performed in human cell culture systems in vitro, to develop a mechanistic mathematical model that can support analysis and prediction of in vivo effects of NPs. The model considers basic cellular mechanisms including proliferation, apoptosis, and production of cytokines in response to NPs. This new model is implemented for macrophages and parameterized using in vitro measurements of changes in cellular viability and mRNA levels of cytokines: TNF, IL-1b, IL-6, IL-8, and IL-10. The model includes in vitro cellular dosimetry due to nanoparticle transport and transformation. Furthermore, the model developed here optimizes the essential cellular parameters based on in vitro measurements, and provides a "stepping stone" for the development of more advanced in vivo models that will incorporate additional cellular and NP interactions.
AU - Mukherjee,D
AU - Royce,SG
AU - Sarkar,S
AU - Thorley,A
AU - Schwander,S
AU - Ryan,MP
AU - Porter,AE
AU - Chung,KF
AU - Tetley,TD
AU - Zhang,J
AU - Georgopoulos,PG
DO - 2014/852890
PY - 2014///
SN - 1687-8205
TI - Modeling in vitro cellular responses to silver nanoparticles
T2 - Journal of Toxicology
UR - http://dx.doi.org/10.1155/2014/852890
UR - http://hdl.handle.net/10044/1/33355
VL - 2014
ER -