Imperial College London

ProfessorMaryRyan

Faculty of EngineeringDepartment of Materials

Vice-Dean (Research), Faculty of Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6755m.p.ryan

 
 
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Location

 

B338Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sarkar:2015:10.1371/journal.pone.0143077,
author = {Sarkar, S and Leo, BF and Carranza, C and Chen, S and Rivas-Santiago, C and Porter, AE and Ryan, MP and Gow, A and Chung, KF and Tetley, TD and Zhang, JJ and Georgopoulos, PG and Ohman-Strickland, PA and Schwander, S},
doi = {10.1371/journal.pone.0143077},
journal = {PLOS One},
title = {Modulation of human macrophage responses to mycobacterium tuberculosis by silver nanoparticles of different size and surface modification},
url = {http://dx.doi.org/10.1371/journal.pone.0143077},
volume = {10},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Exposure to silver nanoparticles (AgNP) used in consumer products carries potential health risks including increased susceptibility to infectious pathogens. Systematic assessments of antimicrobial macrophage immune responses in the context of AgNP exposure are important because uptake of AgNP by macrophages may lead to alterations of innate immune cell functions. In this study we examined the effects of exposure to AgNP with different particle sizes (20 and 110 nm diameters) and surface chemistry (citrate or polyvinlypyrrolidone capping) on cellular toxicity and innate immune responses against Mycobacterium tuberculosis (M.tb) by human monocyte-derived macrophages (MDM). Exposures of MDM to AgNP significantly reduced cellular viability, increased IL8 and decreased IL10 mRNA expression. Exposure of M.tb-infected MDM to AgNP suppressed M.tb-induced expression of IL1B, IL10, and TNFA mRNA. Furthermore, M.tb-induced IL-1β, a cytokine critical for host resistance to M.tb, was inhibited by AgNP but not by carbon black particles indicating that the observed immunosuppressive effects of AgNP are particle specific. Suppressive effects of AgNP on the M.tb-induced host immune responses were in part due to AgNP-mediated interferences with the TLR signaling pathways that culminate in the activation of the transcription factor NF-κB. AgNP exposure suppressed M.tb-induced expression of a subset of NF-κB mediated genes (CSF2, CSF3, IFNG, IL1A, IL1B, IL6, IL10, TNFA, NFKB1A). In addition, AgNP exposure increased the expression of HSPA1A mRNA and the corresponding stress-induced Hsp72 protein. Up-regulation of Hsp72 by AgNP can suppress M.tb-induced NF-κB activation and host immune responses. The observed ability of AgNP to modulate infectious pathogen-induced immune responses has important public health implications.
AU - Sarkar,S
AU - Leo,BF
AU - Carranza,C
AU - Chen,S
AU - Rivas-Santiago,C
AU - Porter,AE
AU - Ryan,MP
AU - Gow,A
AU - Chung,KF
AU - Tetley,TD
AU - Zhang,JJ
AU - Georgopoulos,PG
AU - Ohman-Strickland,PA
AU - Schwander,S
DO - 10.1371/journal.pone.0143077
PY - 2015///
SN - 1932-6203
TI - Modulation of human macrophage responses to mycobacterium tuberculosis by silver nanoparticles of different size and surface modification
T2 - PLOS One
UR - http://dx.doi.org/10.1371/journal.pone.0143077
UR - http://hdl.handle.net/10044/1/33352
VL - 10
ER -