Imperial College London
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ProfessorMaryRyan

Central Faculty, Office of the Provost

Vice-Provost (Research and Enterprise)
 
 
 
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Contact

 

+44 (0)20 7594 6755m.p.ryan

 
 
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Location

 

B338Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Theodorou:2016:10.1080/17435390.2016.1214762,
author = {Theodorou, IG and Ruenraroengsak, P and Gow, A and Schwander, S and Zhang, JJ and Chung, KF and Tetley, TD and Ryan, MP and Porter, AE},
doi = {10.1080/17435390.2016.1214762},
journal = {Nanotoxicology},
pages = {1351--1362},
title = {Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells.},
url = {http://dx.doi.org/10.1080/17435390.2016.1214762},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Inhaled nanoparticles (NPs) have high-deposition rates in the alveolar region of the lung but the effects of pulmonary surfactant (PS) on nanoparticle bioreactivity are unclear. Here, the impact of PS on the stability and dissolution of ZnO nanowires (ZnONWs) was investigated, and linked with their bioreactivity in vitro with human alveolar epithelial type 1-like cells (TT1). Pre-incubation of ZnONWs with Curosurf® (a natural porcine PS) decreased their dissolution at acidic pH, through the formation of a phospholipid corona. Confocal live cell microscopy confirmed that Curosurf® lowered intracellular dissolution, thus delaying the onset of cell death compared to bare ZnONWs. Despite reducing dissolution, Curosurf® significantly increased the uptake of ZnONWs within TT1 cells, ultimately increasing their toxicity after 24 h. Although serum improved ZnONW dispersion in suspension similar to Curosurf®, it had no effect on ZnONW internalization and toxicity, indicating a unique role of PS in promoting particle uptake. In the absence of PS, ZnONW length had no effect on dissolution kinetics or degree of cellular toxicity, indicating a less important role of length in determining ZnONW bioreactivity. This work provides unique findings on the effects of PS on the stability and toxicity of ZnONWs, which could be important in the study of pulmonary toxicity and epithelial-endothelial translocation of nanoparticles in general.
AU  - Theodorou,IG
AU  - Ruenraroengsak,P
AU  - Gow,A
AU  - Schwander,S
AU  - Zhang,JJ
AU  - Chung,KF
AU  - Tetley,TD
AU  - Ryan,MP
AU  - Porter,AE
DO  - 10.1080/17435390.2016.1214762
EP  - 1362
PY  - 2016///
SN  - 1743-5404
SP  - 1351
TI  - Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells.
T2  - Nanotoxicology
UR  - http://dx.doi.org/10.1080/17435390.2016.1214762
UR  - http://hdl.handle.net/10044/1/42588
VL  - 10
ER  -
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