Imperial College London
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DrMariaParaskevaidi

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Research Fellow
 
 
 
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Contact

 

m.paraskevaidi

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bowden:2023,
author = {Bowden, SJ and Ellis, L and Kalliala, I and Paraskevaidi, M and Tighe, J and Kechagias, K and Doulgeraki, T and Paraskevaidis, E and Arbyn, M and Flanagan, J and Veroniki, AA and Kyrgiou, M},
journal = {BMJ Open},
pages = {1--8},
title = {Protocol for a systematic review and meta-analysis of the diagnostic test accuracy of host and HPV DNA methylation in cervical cancer screening and management},
url = {https://bmjopen.bmj.com/content/13/6/e071534},
volume = {13},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Introduction Human papillomavirus (HPV) is necessary but not sufficient for cervical cancer development. During cervical carcinogenesis, methylation levels increase across host and HPV DNA. DNA methylation has been proposed as a test to diagnose cervical intraepithelial neoplasia (CIN); we present a protocol to evaluate the accuracy of methylation markers to detect high-grade CIN and cervical cancer.Methods and analysis We will search electronic databases (Medline, Embase and Cochrane Library), from inception, to identify studies examining DNA methylation as a diagnostic marker for CIN or cervical cancer, in a cervical screening population. The primary outcome will be to assess the diagnostic test accuracy of host and HPV DNA methylation for high-grade CIN; the secondary outcomes will be to examine the accuracy of different methylation cut-off thresholds, and accuracy in high-risk HPV positive women. Our reference standard will be histology. We will perform meta-analyses using Cochrane guidelines for diagnostic test accuracy. We will use the number of true positives, false negatives, true negatives and false positives from individual studies. We will use the bivariate mixed effect model to estimate sensitivity and specificity with 95% CIs; we will employ different bivariate models to estimate sensitivity and specificity at different thresholds if sufficient data per threshold. For insufficient data, the hierarchical summary receiver operating curve model will be used to calculate a summary curve across thresholds. If there is interstudy and intrastudy variation in thresholds, we will use a linear mixed effects model to calculate the optimum threshold. If few studies are available, we will simplify models by assuming no correlation between sensitivity and specificity and perform univariate, random-effects meta-analysis. We will assess the quality of studies using QUADAS-2 and QUADAS-C.Ethics and dissemination Ethical approval is not required. Results will be dissemin
AU  - Bowden,SJ
AU  - Ellis,L
AU  - Kalliala,I
AU  - Paraskevaidi,M
AU  - Tighe,J
AU  - Kechagias,K
AU  - Doulgeraki,T
AU  - Paraskevaidis,E
AU  - Arbyn,M
AU  - Flanagan,J
AU  - Veroniki,AA
AU  - Kyrgiou,M
EP  - 8
PY  - 2023///
SN  - 2044-6055
SP  - 1
TI  - Protocol for a systematic review and meta-analysis of the diagnostic test accuracy of host and HPV DNA methylation in cervical cancer screening and management
T2  - BMJ Open
UR  - https://bmjopen.bmj.com/content/13/6/e071534
UR  - http://hdl.handle.net/10044/1/104626
VL  - 13
ER  -
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