Imperial College London
Alternate

ProfessorMalcolmParker

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Emeritus Professor of Molecular Endocrinology
 
 
 
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Contact

 

+44 (0)20 7594 2177m.parker Website

 
 
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Assistant

 

Miss Kiran Dosanjh +44 (0)20 7594 2176

 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Morganstein:2008:10.1194/jlr.D700029-JLR200,
author = {Morganstein, DL and Christian, M and Turner, JJ and Parker, MG and White, R},
doi = {10.1194/jlr.D700029-JLR200},
journal = {J Lipid Res},
pages = {679--685},
title = {Conditionally immortalized white preadipocytes: a novel adipocyte model},
url = {http://dx.doi.org/10.1194/jlr.D700029-JLR200},
volume = {49},
year = {2008}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - This study describes a novel approach to generate conditionally immortalized preadipocyte cell lines from white adipose tissue (IMWAT) that can be induced to differentiate into white adipocytes even after expansion in culture. Such adipocytes express markers of white fat such as peroxisome proliferator-activated receptor gamma and aP2 but not brown fat markers, have an intact insulin signaling pathway, and express proinflammatory cytokines. They can be readily transduced with adenoviral vectors, allowing them to be used to investigate the consequences of the depletion of specific adipocyte factors using short hairpin RNA. This approach has been used to study the effect of reduced expression of the nuclear receptor corepressor receptor interacting protein 140 (RIP140), a regulator of adipocyte function. The depletion of RIP140 results in changes in metabolic gene expression that resemble those in adipose tissue of the RIP140 null mouse. Thus, IMWAT cells provide a novel model for adipocytes that are derived from preadipocytes rather than fibroblasts and provide an alternative system to primary preadipocytes for the investigation of adipocyte function.
AU  - Morganstein,DL
AU  - Christian,M
AU  - Turner,JJ
AU  - Parker,MG
AU  - White,R
DO  - 10.1194/jlr.D700029-JLR200
EP  - 685
PY  - 2008///
SN  - 0022-2275
SP  - 679
TI  - Conditionally immortalized white preadipocytes: a novel adipocyte model
T2  - J Lipid Res
UR  - http://dx.doi.org/10.1194/jlr.D700029-JLR200
UR  - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18046046
VL  - 49
ER  -
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