Imperial College London

DrMichellePercharde

Faculty of MedicineInstitute of Clinical Sciences

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)20 3313 8235m.percharde Website

 
 
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Location

 

CRB 2007Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

12 results found

Percharde M, Lin CJ, Ramalho-Santos M, 2021, Depletion of nuclear LINE1 RNA in mouse ESCs and embryos, STAR Protocols, Vol: 2

LINE1 is the most active and abundant family of retrotransposons; it is implicated in a number of pathologies, as well as in early embryo development. We present a protocol to specifically knockdown LINE1 in mouse embryonic stem cells and embryos, including details for the nucleofection and zygote microinjection of LINE antisense oligos, followed by RNA FISH validation. This protocol can be used in development, as well as other cell types where LINE1 is believed to be expressed. For complete information on the use and execution of this protocol, please refer to Percharde et al. (2018).

Journal article

Lu JY, Chang L, Li T, Wang T, Yin Y, Zhan G, Han X, Zhang K, Tao Y, Percharde M, Wang L, Peng Q, Yan P, Zhang H, Bi X, Shao W, Hong Y, Wu Z, Ma R, Wang P, Li W, Zhang J, Chang Z, Hou Y, Zhu B, Ramalho-Santos M, Li P, Xie W, Na J, Sun Y, Shen Xet al., 2021, Homotypic clustering of L1 and B1/Alu repeats compartmentalizes the 3D genome, CELL RESEARCH, Vol: 31, Pages: 613-630, ISSN: 1001-0602

Journal article

Kuwahara A, Lewis AE, Coombes C, Leung F-S, Percharde M, Bush JOet al., 2020, Delineating the early transcriptional specification of the mammalian trachea and esophagus, eLife, Vol: 9

<jats:p>The genome-scale transcriptional programs that specify the mammalian trachea and esophagus are unknown. Though NKX2-1 and SOX2 are hypothesized to be co-repressive master regulators of tracheoesophageal fates, this is untested at a whole transcriptomic scale and their downstream networks remain unidentified. By combining single-cell RNA-sequencing with bulk RNA-sequencing of Nkx2-1 mutants and NKX2-1 ChIP-sequencing in mouse embryos, we delineate the NKX2-1 transcriptional program in tracheoesophageal specification, and discover that the majority of the tracheal and esophageal transcriptome is NKX2-1 independent. To decouple the NKX2-1 transcriptional program from regulation by SOX2, we interrogate the expression of newly-identified tracheal and esophageal markers in Sox2/Nkx2-1 compound mutants. Finally, we discover that NKX2-1 binds directly to Shh and Wnt7b and regulates their expression to control mesenchymal specification to cartilage and smooth muscle, coupling epithelial identity with mesenchymal specification. These findings create a new framework for understanding early tracheoesophageal fate specification at the genome-wide level.</jats:p>

Journal article

Lu JY, Shao W, Chang L, Yin Y, Li T, Zhang H, Hong Y, Percharde M, Guo L, Wu Z, Liu L, Liu W, Yan P, Ramalho-Santos M, Sun Y, Shen Xet al., 2020, Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation, CELL REPORTS, Vol: 30, Pages: 3296-+, ISSN: 2211-1247

Journal article

Percharde M, Lin C-J, Yin Y, Guan J, Peixoto GA, Bulut-Karslioglu A, Biechele S, Huang B, Shen X, Ramalho-Santos Met al., 2018, A LINE1-Nucleolin Partnership Regulates Early Development and ESC Identity, CELL, Vol: 174, Pages: 391-+, ISSN: 0092-8674

Journal article

Bulut-Karslioglu A, Macrae TA, Oses-Prieto JA, Covarrubias S, Percharde M, Ku G, Diaz A, McManus MT, Burlingame AL, Ramalho-Santos Met al., 2018, The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output, CELL STEM CELL, Vol: 22, Pages: 369-+, ISSN: 1934-5909

Journal article

Percharde M, Wong P, Ramalho-Santos M, 2017, Global Hypertranscription in the Mouse Embryonic Germline, CELL REPORTS, Vol: 19, Pages: 1987-1996, ISSN: 2211-1247

Journal article

Percharde M, Bulut-Karslioglu A, Ramalho-Santos M, 2017, Hypertranscription in Development, Stem Cells, and Regeneration, DEVELOPMENTAL CELL, Vol: 40, Pages: 9-21, ISSN: 1534-5807

Journal article

Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song JS, Ramalho-Santos Met al., 2016, YAP Induces Human Naive Pluripotency, CELL REPORTS, Vol: 14, Pages: 2301-2312, ISSN: 2211-1247

Journal article

Percharde M, Azuara V, 2013, Essential roles for the nuclear receptor coactivator Ncoa3 in pluripotency, CELL CYCLE, Vol: 12, Pages: 195-196, ISSN: 1538-4101

Journal article

Percharde M, Lavial F, Ng J-H, Kumar V, Tomaz RA, Martin N, Yeo J-C, Gil J, Prabhakar S, Ng H-H, Parker MG, Azuara Vet al., 2012, Ncoa3 functions as an essential Esrrb coactivator to sustain embryonic stem cell self-renewal and reprogramming, GENES & DEVELOPMENT, Vol: 26, Pages: 2286-2298, ISSN: 0890-9369

Journal article

Lopez J, Percharde M, Coley HM, Webb A, Crook Tet al., 2009, The context and potential of epigenetics in oncology, BRITISH JOURNAL OF CANCER, Vol: 100, Pages: 571-577, ISSN: 0007-0920

Journal article

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