Imperial College London
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DrMichellePercharde

Faculty of Medicine, Institute of Clinical Sciences

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 3313 8299m.percharde Website

 
 
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Location

 

CRB 2006 / 2013Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lu:2019:10.1101/802173,
author = {Lu, JY and Chang, L and Li, T and Wang, T and Yin, Y and Zhan, G and Zhang, K and Percharde, M and Wang, L and Peng, Q and Yan, P and Zhang, H and Han, X and Bi, X and Shao, W and Hong, Y and Wu, Z and Wang, P and Li, W and Zhang, J and Chang, Z and Hou, Y and Li, P and Ramalho-Santos, M and Na, J and Xie, W and Sun, Y and Shen, X},
doi = {10.1101/802173},
title = {L1 and B1 repeats blueprint the spatial organization of chromatin},
url = {http://dx.doi.org/10.1101/802173},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>SUMMARY</jats:title><jats:p>Despite extensive mapping of three-dimensional (3D) chromatin structures, the basic principles underlying genome folding remain unknown. Here, we report a fundamental role for L1 and B1 retrotransposons in shaping the macroscopic 3D genome structure. Homotypic clustering of B1 and L1 repeats in the nuclear interior or at the nuclear and nucleolar peripheries, respectively, segregates the genome into mutually exclusive nuclear compartments. This spatial segregation of L1 and B1 is conserved in mouse and human cells, and occurs dynamically during establishment of the 3D chromatin structure in early embryogenesis and the cell cycle. Depletion of L1 transcripts drastically disrupts the spatial distributions of L1- and B1-rich compartments. L1 transcripts are strongly associated with L1 DNA sequences and induce phase separation of the heterochromatin protein HP1α. Our results suggest that genomic repeats act as the blueprint of chromatin macrostructure, thus explaining the conserved higher-order structure of chromatin across mammalian cells.</jats:p>
AU  - Lu,JY
AU  - Chang,L
AU  - Li,T
AU  - Wang,T
AU  - Yin,Y
AU  - Zhan,G
AU  - Zhang,K
AU  - Percharde,M
AU  - Wang,L
AU  - Peng,Q
AU  - Yan,P
AU  - Zhang,H
AU  - Han,X
AU  - Bi,X
AU  - Shao,W
AU  - Hong,Y
AU  - Wu,Z
AU  - Wang,P
AU  - Li,W
AU  - Zhang,J
AU  - Chang,Z
AU  - Hou,Y
AU  - Li,P
AU  - Ramalho-Santos,M
AU  - Na,J
AU  - Xie,W
AU  - Sun,Y
AU  - Shen,X
DO  - 10.1101/802173
PY  - 2019///
TI  - L1 and B1 repeats blueprint the spatial organization of chromatin
UR  - http://dx.doi.org/10.1101/802173
ER  -
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