Imperial College London
Dr Marie-Christine Ramel

DrMarie-ChristineRamel

Faculty of Natural Sciences, Department of Life Sciences

Visiting Researcher
 
 
 
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Contact

 

+44 (0)20 7594 5405m.ramel

 
 
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Location

 

6150Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ramel:2005:10.1016/j.ydbio.2005.08.012,
author = {Ramel, M-C and Buckles, GR and Baker, KD and Lekven, AC},
doi = {10.1016/j.ydbio.2005.08.012},
journal = {Dev Biol},
pages = {237--248},
title = {WNT8 and BMP2B co-regulate non-axial mesoderm patterning during zebrafish gastrulation.},
url = {http://dx.doi.org/10.1016/j.ydbio.2005.08.012},
volume = {287},
year = {2005}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - During vertebrate mesoderm formation, fates are established according to position in the dorsoventral (D/V) axis of the embryo. Initially, maternal signaling divides nascent mesoderm into axial (dorsal) and non-axial (ventral) domains. Although the subsequent subdivision of non-axial mesoderm into multiple D/V fate domains is known to involve zygotic Wnt8 and BMP signaling as well as the Vent/Vox/Ved family of transcriptional repressors, how levels of signaling activity are translated into differential regulation of fates is not well understood. To address this question, we have analyzed zebrafish embryos lacking Wnt8 and BMP2b. Zebrafish wnt8; swr (bmp2b) double mutants display a progressive loss of non-axial mesoderm and a concomitant expansion of axial mesoderm during gastrulation. Mesoderm induction and specification of the axial domain occur normally in wnt8; swr mutants, but dorsal mesoderm genes eventually come to be expressed throughout the mesoderm, suggesting that the establishment of non-axial mesoderm identity requires continual repression of dorsal mesoderm factors, including repressors of ventral genes. Loss-of-function for Vent, Vox, and Ved phenocopies the wnt8; swr mutant phenotype, consistent with Wnt8 and BMP2b maintaining non-axial mesoderm identity during gastrulation through the regulation of these three transcriptional repressors. We postulate that timely differentiation of the mesoderm requires the maintenance of non-axial mesoderm identity by Wnt8 and BMP2b at the onset of gastrulation followed by subdivision of the non-axial mesoderm into different functional domains during gastrulation.
AU  - Ramel,M-C
AU  - Buckles,GR
AU  - Baker,KD
AU  - Lekven,AC
DO  - 10.1016/j.ydbio.2005.08.012
EP  - 248
PY  - 2005///
SN  - 0012-1606
SP  - 237
TI  - WNT8 and BMP2B co-regulate non-axial mesoderm patterning during zebrafish gastrulation.
T2  - Dev Biol
UR  - http://dx.doi.org/10.1016/j.ydbio.2005.08.012
UR  - https://www.ncbi.nlm.nih.gov/pubmed/16216234
VL  - 287
ER  -
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