Imperial College London
Alternate

Emeritus ProfessorMaryRitter

Faculty of Medicine, Department of Immunology and Inflammation

Emeritus Professor of Immunology
 
 
 
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Contact

 

+44 (0)20 7492 1921m.ritter

 
 
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Location

 

31-35 Kirby StreetLondon EC1N 8TE

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Summary

 

Publications

Citation

BibTex format

@article{Imami:1998:1998/29340,
author = {Imami, N and Ladyman, HM and Vincents, B and al-Tubuly, A and Freysdóttir, J and Sedibane, ML and Taylor-Fishwick, DA and Foxwell, BM and Ritter, MA},
doi = {1998/29340},
journal = {Developmental Immunology},
pages = {41--52},
title = {K21-antigen: a molecule shared by the microenvironments of the human thymus and germinal centers},
url = {http://dx.doi.org/10.1155/1998/29340},
volume = {6},
year = {1998}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The mouse IgG1 monoclonal antibody (mAb) K21 recognizes a 230-kD molecule (K21-Ag) on Hassall's corpuscles in the human thymus. This mAb also stains cultured thymic epithelial cells as well as other epithelial cell lines, revealing a predominant intracellular localization. Further analysis with mAb K21 on other lymphoid tissues showed that it also stains cells within the germinal centers of human tonsils, both lymphoid (B) cells and some with the appearance of follicular dendritic cells. Double immunostaining of tonsil sections shows that K21-Ag is not expressed by T cells, whereas staining with anti-CD22 and -CD23 mAb revealed some doublepositive cells. A subpopulation of the lymphoid cells express the K21-Ag much more strongly. This K21++/CD23++ subpopulation of cells is localized in the apical light zone of germinal centers, suggesting that K21-Ag may be an important marker for the selected centrocytes within germinal centers and may play a role in B-cell selection and/or development of B-cell memory. Flow cytometric analysis showed that K21-Ag is expressed on the surface of a very low percentage of thymocytes, tonsillar lymphocytes, and peripheral blood mononuclear cells. Analysis of purified/separated tonsillar T and B lymphocytes showed that T cells do not express the K21-Ag; in contrast, B cells express low levels of the K21-Ag, and this together with CD23 is upregulated after mitogenic stimulation. Our data therefore raise the possibility that the K2l- Ag may play a role in B-lymphocyte activation/selection.
AU  - Imami,N
AU  - Ladyman,HM
AU  - Vincents,B
AU  - al-Tubuly,A
AU  - Freysdóttir,J
AU  - Sedibane,ML
AU  - Taylor-Fishwick,DA
AU  - Foxwell,BM
AU  - Ritter,MA
DO  - 1998/29340
EP  - 52
PY  - 1998///
SN  - 1044-6672
SP  - 41
TI  - K21-antigen: a molecule shared by the microenvironments of the human thymus and germinal centers
T2  - Developmental Immunology
UR  - http://dx.doi.org/10.1155/1998/29340
UR  - http://hdl.handle.net/10044/1/32987
VL  - 6
ER  -
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