Imperial College London
Alternate

ProfessorMichaelSeckl

Faculty of Medicine, Department of Surgery & Cancer

Professor of Molecular Cancer Medicine
 
 
 
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Contact

 

+44 (0)20 3311 1421m.seckl

 
 
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Location

 

08Cyclotron buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pardo:2016:10.15252/embr.201540970,
author = {Pardo, OE and Munro, CE and Castellano, L and Hu, Y and Mauri, F and Krell, J and Lara, R and Pinho, FG and Pellegrino, L and Pshezhetskiy, D and Wang, Y and Waxman, J and Seckl, MJ and Stebbing, J},
doi = {10.15252/embr.201540970},
journal = {EMBO Reports},
pages = {570--584},
title = {miR-515-5p controls cancer cell migration through MARK4 regulation},
url = {http://dx.doi.org/10.15252/embr.201540970},
volume = {17},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Here we show that miR-515-5p inhibits cancer cell migration and metastasis. RNA-seqanalyses of both estrogen receptor-positive and negative breast cancer cells overexpressingmiR-515-5p reveals down-regulation of NRAS, FZD4, CDC42BPA, PIK3C2Band MARK4 mRNAs. We demonstrate that miR-515-5p inhibits MARK4 directly 3’UTRinteraction and that MARK4 knockdown mimics the effect of miR-515-5p on breast andlung cancer cell migration. MARK4 over-expression rescues the inhibitory effects of miR-515-5p, suggesting miR-515-5p mediates this process through MARK4 down-regulation.Furthermore, miR-515-5p expression is reduced in metastases compared to primarytumours derived from both in vivo xenografts and samples from patients with breastcancer. Conversely, miR-515-5p overexpression prevents tumour cell dissemination ina mouse metastatic model. Moreover, high miR-515-5p and low MARK4 expressioncorrelate with increased breast and lung cancer patients’ survival, respectively. Takentogether, these data demonstrate the importance of miR-515-5p/MARK4 regulation incell migration and metastasis across two common cancers.
AU  - Pardo,OE
AU  - Munro,CE
AU  - Castellano,L
AU  - Hu,Y
AU  - Mauri,F
AU  - Krell,J
AU  - Lara,R
AU  - Pinho,FG
AU  - Pellegrino,L
AU  - Pshezhetskiy,D
AU  - Wang,Y
AU  - Waxman,J
AU  - Seckl,MJ
AU  - Stebbing,J
DO  - 10.15252/embr.201540970
EP  - 584
PY  - 2016///
SN  - 1469-221X
SP  - 570
TI  - miR-515-5p controls cancer cell migration through MARK4 regulation
T2  - EMBO Reports
UR  - http://dx.doi.org/10.15252/embr.201540970
UR  - http://hdl.handle.net/10044/1/29024
VL  - 17
ER  -
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