Imperial College London
Alternate

Emeritus ProfessorMurraySelkirk

Faculty of Natural Sciences, Department of Life Sciences

Emeritus Professor in Molecular Parasitology
 
 
 
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Contact

 

+44 (0)20 7594 5214m.selkirk Website

 
 
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Location

 

204Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Berkachy:2021:10.1016/j.ijpara.2020.07.011,
author = {Berkachy, R and Smyth, DJ and Schnoeller, C and Harcus, Y and Maizels, RM and Selkirk, ME and Gounaris, K},
doi = {10.1016/j.ijpara.2020.07.011},
journal = {International Journal for Parasitology},
pages = {39--48},
title = {Characterisation of the secreted apyrase family of Heligmosomoides polygyrus},
url = {http://dx.doi.org/10.1016/j.ijpara.2020.07.011},
volume = {51},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Apyrases are a recurrent feature of secretomes from numerous species of parasitic nematodes. Here we characterise the five apyrases secreted by Heligmosomoides polygyrus, a natural parasite of mice and a widely used laboratory model for intestinal nematode infection. All five enzymes are closely related to soluble calcium-activated nucleotidases described in a variety of organisms, and distinct from the CD39 family of ecto-nucleotidases. Expression is maximal in adult worms and restricted to adults and L4s. Recombinant apyrases were produced and purified from Pichia pastoris. The five enzymes showed very similar biochemical properties, with strict calcium dependence and a broad substrate specificity, catalysing the hydrolysis of all nucleoside tri- and diphosphates, with no activity against nucleoside monophosphates. Natural infection of mice provoked very low antibodies to any enzyme, but immunisation with an apyrase cocktail showed partial protection against reinfection, with reduced egg output and parasite recovery. The most likely role for nematode secreted apyrases is hydrolysis of extracellular ATP, which acts as an alarmin for cellular release of IL-33 and initiation of type 2 immunity.
AU  - Berkachy,R
AU  - Smyth,DJ
AU  - Schnoeller,C
AU  - Harcus,Y
AU  - Maizels,RM
AU  - Selkirk,ME
AU  - Gounaris,K
DO  - 10.1016/j.ijpara.2020.07.011
EP  - 48
PY  - 2021///
SN  - 0020-7519
SP  - 39
TI  - Characterisation of the secreted apyrase family of Heligmosomoides polygyrus
T2  - International Journal for Parasitology
UR  - http://dx.doi.org/10.1016/j.ijpara.2020.07.011
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32931780
UR  - http://hdl.handle.net/10044/1/85749
VL  - 51
ER  -
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