Imperial College London
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Professor Michael A. ("Mike") Skinner

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor in Virology
 
 
 
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Contact

 

+44 (0)20 7594 3938m.skinner Website

 
 
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Assistant

 

Mrs Yasmin Mallu +44 (0)20 7594 3972

 
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Location

 

315Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Giotis:2020:10.3390/biomedicines8120634,
author = {Giotis, E and Laidlaw, S and Bidgood, S and Albrecht, D and Burden, J and Robey, R and Mercer, J and Skinner, M},
doi = {10.3390/biomedicines8120634},
journal = {Biomedicines},
pages = {1--21},
title = {Modulation of early host innate immune response by an avipox vaccine virus’ lateral body protein},
url = {http://dx.doi.org/10.3390/biomedicines8120634},
volume = {8},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The avian pathogen fowlpox virus (FWPV) has been successfully used as a vaccine vector in poultry and humans, but relatively little is known about its ability to modulate host antiviral immune responses in these hosts, which are replication-permissive and nonpermissive, respectively. FWPV is highly resistant to avian type I interferon (IFN) and able to completely block the host IFN-response. Microarray screening of host IFN-regulated gene expression in cells infected with 59 different, nonessential FWPV gene knockout mutants revealed that FPV184 confers immunomodulatory capacity. We report that the FPV184-knockout virus (FWPVΔ184) induces the cellular IFN response as early as 2 h postinfection. The wild-type, uninduced phenotype can be rescued by transient expression of FPV184 in FWPVΔ184-infected cells. Ectopic expression of FPV184 inhibited polyI:C activation of the chicken IFN-β promoter and IFN-α activation of the chicken Mx1 promoter. Confocal and correlative super-resolution light and electron microscopy demonstrated that FPV184 has a functional nuclear localisation signal domain and is packaged in the lateral bodies of the virions. Taken together, these results provide a paradigm for a late poxvirus structural protein packaged in the lateral bodies, capable of suppressing IFN induction early during the next round of infection.
AU  - Giotis,E
AU  - Laidlaw,S
AU  - Bidgood,S
AU  - Albrecht,D
AU  - Burden,J
AU  - Robey,R
AU  - Mercer,J
AU  - Skinner,M
DO  - 10.3390/biomedicines8120634
EP  - 21
PY  - 2020///
SN  - 2227-9059
SP  - 1
TI  - Modulation of early host innate immune response by an avipox vaccine virus’ lateral body protein
T2  - Biomedicines
UR  - http://dx.doi.org/10.3390/biomedicines8120634
UR  - https://www.mdpi.com/2227-9059/8/12/634
UR  - http://hdl.handle.net/10044/1/85101
VL  - 8
ER  -
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