Imperial College London
Alternate

ProfessorMichaelSternberg

Faculty of Natural Sciences, Department of Life Sciences

Director, Systems Biology and Bioinformatics Centre
 
 
 
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Contact

 

+44 (0)20 7594 5212m.sternberg Website

 
 
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Location

 

306Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kelley:2015:10.1038/nprot.2015.053,
author = {Kelley, LA and Mezulis, S and Yates, CM and Wass, MN and Sternberg, MJ},
doi = {10.1038/nprot.2015.053},
journal = {Nature Protocols},
pages = {845--858},
title = {The Phyre2 web portal for protein modeling, prediction and analysis},
url = {http://dx.doi.org/10.1038/nprot.2015.053},
volume = {10},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Phyre2 is a suite of tools available on the web to predict and analyze protein structure, function and mutations. The focus of Phyre2 is to provide biologists with a simple and intuitive interface to state-of-the-art protein bioinformatics tools. Phyre2 replaces Phyre, the original version of the server for which we previously published a paper in Nature Protocols. In this updated protocol, we describe Phyre2, which uses advanced remote homology detection methods to build 3D models, predict ligand binding sites and analyze the effect of amino acid variants (e.g., nonsynonymous SNPs (nsSNPs)) for a user's protein sequence. Users are guided through results by a simple interface at a level of detail they determine. This protocol will guide users from submitting a protein sequence to interpreting the secondary and tertiary structure of their models, their domain composition and model quality. A range of additional available tools is described to find a protein structure in a genome, to submit large number of sequences at once and to automatically run weekly searches for proteins that are difficult to model. The server is available at http://www.sbg.bio.ic.ac.uk/phyre2. A typical structure prediction will be returned between 30 min and 2 h after submission.
AU  - Kelley,LA
AU  - Mezulis,S
AU  - Yates,CM
AU  - Wass,MN
AU  - Sternberg,MJ
DO  - 10.1038/nprot.2015.053
EP  - 858
PY  - 2015///
SN  - 1754-2189
SP  - 845
TI  - The Phyre2 web portal for protein modeling, prediction and analysis
T2  - Nature Protocols
UR  - http://dx.doi.org/10.1038/nprot.2015.053
UR  - https://www.nature.com/articles/nprot.2015.053
UR  - http://hdl.handle.net/10044/1/21823
VL  - 10
ER  -
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