Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
//

Contact

 

+44 (0)20 7594 6804m.stevens

 
 
//

Location

 

208Royal School of MinesSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Li:2020:10.1039/c9bm01829h,
author = {Li, S and Tallia, F and Mohammed, AA and Stevens, MM and Jones, JR},
doi = {10.1039/c9bm01829h},
journal = {Biomaterials Science},
pages = {4458--4466},
title = {Scaffold channel size influences stem cell differentiation pathway in 3-D printed silica hybrid scaffolds for cartilage regeneration},
url = {http://dx.doi.org/10.1039/c9bm01829h},
volume = {8},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We report that 3-D printed scaffold channel size can direct bone marrow derived stem cell differentiation. Treatment of articular cartilage trauma injuries, such as microfracture surgery, have limited success because durability is limited as fibrocartilage forms. A scaffold-assisted approach, combining microfracture with biomaterials has potential if the scaffold can promote articular cartilage production and share load with cartilage. Here, we investigated human bone marrow derived stromal cell (hBMSC) differentiation in vitro in 3-D printed silica/poly(tetrahydrofuran)/poly(ε-caprolactone) hybrid scaffolds with specific channel sizes. Channel widths of ∼230 μm (210 ± 22 μm mean strut size, 42.4 ± 3.9% porosity) provoked hBMSC differentiation down a chondrogenic path, with collagen Type II matrix prevalent, indicative of hyaline cartilage. When pores were larger (∼500 μm, 229 ± 29 μm mean strut size, 63.8 ± 1.6% porosity) collagen Type I was dominant, indicating fibrocartilage. There was less matrix and voids in smaller channels (∼100 μm, 218 ± 28 μm mean strut size, 31.2 ± 2.9% porosity). Our findings suggest that a 200–250 μm pore channel width, in combination with the surface chemistry and stiffness of the scaffold, is optimal for cell–cell interactions to promote chondrogenic differentiation and enable the chondrocytes to maintain their phenotype.
AU  - Li,S
AU  - Tallia,F
AU  - Mohammed,AA
AU  - Stevens,MM
AU  - Jones,JR
DO  - 10.1039/c9bm01829h
EP  - 4466
PY  - 2020///
SN  - 2047-4830
SP  - 4458
TI  - Scaffold channel size influences stem cell differentiation pathway in 3-D printed silica hybrid scaffolds for cartilage regeneration
T2  - Biomaterials Science
UR  - http://dx.doi.org/10.1039/c9bm01829h
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/BM/C9BM01829H#!divAbstract
UR  - http://hdl.handle.net/10044/1/77047
VL  - 8
ER  -
Download