Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kauscher:2020:10.1021/acs.langmuir.9b03479,
author = {Kauscher, U and Pender, J and Nagelkerke, A and Holme, M and Nele, V and Massi, L and Gopal, S and Whittaker, T and Stevens, M},
doi = {10.1021/acs.langmuir.9b03479},
journal = {Langmuir: the ACS journal of surfaces and colloids},
pages = {3912--3923},
title = {Gold nanocluster extracellular vesicle supraparticles: Self-assembled nanostructures for 3D uptake visualization},
url = {http://dx.doi.org/10.1021/acs.langmuir.9b03479},
volume = {36},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Extracellular vesicles (EVs) are secreted by the vast majority of cells and are being intensively studied due to their emerging involvement in a variety of cellular communication processes. However, the study of their cellular uptake and fate has been hampered by difficulty in imaging EVs against the cellular background. Here, we show that EVs combined with hydrophobic gold nanoclusters (AuNCs) can self-assemble into supraparticles, offering an excellent labeling strategy for high-resolution electron microscopic imaging in vitro. We have tracked and visualized the reuptake of breast cancer cell-derived EV AuNC supraparticles into their parent cells, from early endocytosis to lysosomal degradation, using focused ion beam-scanning electron microscopy (FIB-SEM). The presence of gold within the EVs and lysosomes was confirmed via DF-STEM EDX analysis of lift-out sections. The demonstrated formation of AuNC EV supraparticles will facilitate future applications in EV imaging as well as the EV-assisted cellular delivery of AuNCs.
AU  - Kauscher,U
AU  - Pender,J
AU  - Nagelkerke,A
AU  - Holme,M
AU  - Nele,V
AU  - Massi,L
AU  - Gopal,S
AU  - Whittaker,T
AU  - Stevens,M
DO  - 10.1021/acs.langmuir.9b03479
EP  - 3923
PY  - 2020///
SN  - 0743-7463
SP  - 3912
TI  - Gold nanocluster extracellular vesicle supraparticles: Self-assembled nanostructures for 3D uptake visualization
T2  - Langmuir: the ACS journal of surfaces and colloids
UR  - http://dx.doi.org/10.1021/acs.langmuir.9b03479
UR  - https://pubs.acs.org/doi/10.1021/acs.langmuir.9b03479
UR  - http://hdl.handle.net/10044/1/78710
VL  - 36
ER  -
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