Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kim:2021:10.1002/adma.202007738,
author = {Kim, N and Kim, E and Kim, H and Thomas, M and Najer, A and Stevens, M},
doi = {10.1002/adma.202007738},
journal = {Advanced Materials},
pages = {1--10},
title = {Tumor-targeting cholesterol-decorated DNA nanoflowers for intracellular ratiometric aptasensing},
url = {http://dx.doi.org/10.1002/adma.202007738},
volume = {33},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Probing endogenous molecular profiles is of fundamental importance to understand cellular function and processes. Despite the promise of programmable nucleicacidbased aptasensors across the breadth of biomolecular detection, targetresponsive aptasensors enabling intracellular detection are as of yet infrequently realized. Several challenges remain, including the difficulties in quantification/normalization of quencherbased intensiometric signals, stability issues of the probe architecture, and complex sensor operations often necessitating extensive structural modeling. Here, the biomimetic crystallizationempowered selfassembly of a tumortargetable DNA–inorganic hybrid nanocomposite aptasensor is presented, which enables Förster resonance energy transfer (FRET)based quantitative interpretation of changes in the cellular target abundance. Leveraging the design programmability and highthroughput fabrication of rolling circle amplificationdriven DNA nanoarchitecture, this designer platform offers a method to selfassemble a robust nanosensor from a multifunctionalityencoded template that includes a celltargeting aptamer, a ratiometric aptasensor, and a cholesteroldecorating element. Taking prostate cancer cells and intracellular adenosine triphosphate molecules as a model system, a synergistic effect in the targeted delivery by cholesterol and aptamers, and the feasibility of quantitative intracellular aptasensing are demonstrated. It is envisioned that this approach provides a highly generalizable strategy across wideranging target systems toward a biologically deliverable nanosensor that enables quantitative monitoring of the abundance of endogenous biomolecules.
AU  - Kim,N
AU  - Kim,E
AU  - Kim,H
AU  - Thomas,M
AU  - Najer,A
AU  - Stevens,M
DO  - 10.1002/adma.202007738
EP  - 10
PY  - 2021///
SN  - 0935-9648
SP  - 1
TI  - Tumor-targeting cholesterol-decorated DNA nanoflowers for intracellular ratiometric aptasensing
T2  - Advanced Materials
UR  - http://dx.doi.org/10.1002/adma.202007738
UR  - https://onlinelibrary.wiley.com/doi/10.1002/adma.202007738
UR  - http://hdl.handle.net/10044/1/86511
VL  - 33
ER  -
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