Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Maynard:2021:10.1038/s41598-021-86315-x,
author = {Maynard, SA and Pchelintseva, E and Zwi-Dantsis, L and Nagelkerke, A and Gopal, S and Korchev, YE and Shevchuk, A and Stevens, M},
doi = {10.1038/s41598-021-86315-x},
journal = {Scientific Reports},
pages = {1--14},
title = {IL-1β mediated nanoscale surface clustering of integrin α5β1 regulates the adhesion of mesenchymal stem cells},
url = {http://dx.doi.org/10.1038/s41598-021-86315-x},
volume = {11},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Clinical use of human mesenchymal stem cells (hMSCs) is limited due to their rapid clearance, reducing their therapeutic efficacy. The inflammatory cytokine IL-1β activates hMSCs and is known to enhance their engraftment. Consequently, understanding the molecular mechanism of this inflammation-triggered adhesion is of great clinical interest to improving hMSC retention at sites of tissue damage. Integrins are cell–matrix adhesion receptors, and clustering of integrins at the nanoscale underlies cell adhesion. Here, we found that IL-1β enhances adhesion of hMSCs via increased focal adhesion contacts in an α5β1 integrin-specific manner. Further, through quantitative super-resolution imaging we elucidated that IL-1β specifically increases nanoscale integrin α5β1 availability and clustering at the plasma membrane, whilst conserving cluster area. Taken together, these results demonstrate that hMSC adhesion via IL-1β stimulation is partly regulated through integrin α5β1 spatial organization at the cell surface. These results provide new insight into integrin clustering in inflammation and provide a rational basis for design of therapies directed at improving hMSC engraftment.
AU  - Maynard,SA
AU  - Pchelintseva,E
AU  - Zwi-Dantsis,L
AU  - Nagelkerke,A
AU  - Gopal,S
AU  - Korchev,YE
AU  - Shevchuk,A
AU  - Stevens,M
DO  - 10.1038/s41598-021-86315-x
EP  - 14
PY  - 2021///
SN  - 2045-2322
SP  - 1
TI  - IL-1β mediated nanoscale surface clustering of integrin α5β1 regulates the adhesion of mesenchymal stem cells
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-021-86315-x
UR  - https://www.nature.com/articles/s41598-021-86315-x
UR  - http://hdl.handle.net/10044/1/88557
VL  - 11
ER  -
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