Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Richards:2021:10.1039/D1NR02584H,
author = {Richards, DA and Thomas, M and Szijj, P and Foote, J and Chen, Y and Nogueira, CF and Chudasama, V and Stevens, M},
doi = {10.1039/D1NR02584H},
journal = {Nanoscale},
pages = {11921--11931},
title = {Employing defined bioconjugates to generate chemically functionalised gold nanoparticles for in vitro diagnostic applications},
url = {http://dx.doi.org/10.1039/D1NR02584H},
volume = {13},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Novel methods for introducing chemical and biological functionality to the surface of gold nanoparticles serve to increase the utility of this class of nanomaterials across a range of applications. To date, methods for functionalising gold surfaces have relied upon uncontrollable non-specific adsorption, bespoke chemical linkers, or non-generalisable protein–protein interactions. Herein we report a versatile method for introducing functionality to gold nanoparticles by exploiting the strong interaction between chemically functionalised bovine serum albumin (f-BSA) and citrate-capped gold nanoparticles (AuNPs). We establish the generalisability of the method by introducing a variety of functionalities to gold nanoparticles using cheap, commercially available chemical linkers. The utility of this approach is further demonstrated through the conjugation of the monoclonal antibody Ontruzant to f-BSA–AuNPs using inverse electron-demand Diels–Alder (iEDDA) click chemistry, a hitherto unexplored chemistry for AuNP–IgG conjugation. Finally, we show that the AuNP–Ontruzant particles generated via f-BSA–AuNPs have a greater affinity for their target in a lateral flow format when compared to conventional physisorption, highlighting the potential of this technology for producing sensitive diagnostic tests.
AU  - Richards,DA
AU  - Thomas,M
AU  - Szijj,P
AU  - Foote,J
AU  - Chen,Y
AU  - Nogueira,CF
AU  - Chudasama,V
AU  - Stevens,M
DO  - 10.1039/D1NR02584H
EP  - 11931
PY  - 2021///
SN  - 2040-3364
SP  - 11921
TI  - Employing defined bioconjugates to generate chemically functionalised gold nanoparticles for in vitro diagnostic applications
T2  - Nanoscale
UR  - http://dx.doi.org/10.1039/D1NR02584H
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/NR/D1NR02584H#!divAbstract
UR  - http://hdl.handle.net/10044/1/90600
VL  - 13
ER  -
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