Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Barriga:2022:10.1002/adma.202200839,
author = {Barriga, HMG and Pence, IJ and Holme, M and Doutch, J and Penders, J and Nele, V and Thomas, M and Carroni, M and Stevens, M},
doi = {10.1002/adma.202200839},
journal = {Advanced Materials},
pages = {1--11},
title = {Coupling lipid nanoparticle structure and automated single particle composition analysis to design phospholipase responsive nanocarriers},
url = {http://dx.doi.org/10.1002/adma.202200839},
volume = {34},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Lipid nanoparticles (LNPs) are versatile structures with tunable physicochemical properties that are ideally suited as a platform for vaccine delivery and RNA therapeutics. A key barrier to LNP rational design is the inability to relate composition and structure to intracellular processing and function. Here we combine Single Particle Automated Raman Trapping Analysis (SPARTA®) with small angle scattering (SAXS / SANS) techniques to link LNP composition with internal structure and morphology and to monitor dynamic LNP - phospholipase D (PLD) interactions. Our analysis demonstrates that phospholipase D, a key intracellular trafficking mediator, can access the entire LNP lipid membrane to generate stable, anionic LNPs. PLD activity on vesicles with matched amounts of enzyme substrate was an order of magnitude lower, indicating that the LNP lipid membrane structure can be used to control enzyme interactions. This represents an opportunity to design enzyme-responsive LNP solutions for stimuli-responsive delivery and diseases where PLD is dysregulated.
AU  - Barriga,HMG
AU  - Pence,IJ
AU  - Holme,M
AU  - Doutch,J
AU  - Penders,J
AU  - Nele,V
AU  - Thomas,M
AU  - Carroni,M
AU  - Stevens,M
DO  - 10.1002/adma.202200839
EP  - 11
PY  - 2022///
SN  - 0935-9648
SP  - 1
TI  - Coupling lipid nanoparticle structure and automated single particle composition analysis to design phospholipase responsive nanocarriers
T2  - Advanced Materials
UR  - http://dx.doi.org/10.1002/adma.202200839
UR  - https://onlinelibrary.wiley.com/doi/10.1002/adma.202200839
UR  - http://hdl.handle.net/10044/1/98592
VL  - 34
ER  -
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