Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Utterström:2022:10.1021/acs.bioconjchem.2c00115,
author = {Utterström, J and Barriga, HMG and Holme, MN and Selegård, R and Stevens, MM and Aili, D},
doi = {10.1021/acs.bioconjchem.2c00115},
journal = {Bioconjugate Chemistry},
pages = {736--746},
title = {Peptide-folding triggered phase separation and lipid membrane destabilization in cholesterol-rich lipid vesicles.},
url = {http://dx.doi.org/10.1021/acs.bioconjchem.2c00115},
volume = {33},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Liposome-based drug delivery systems are widely used to improve drug pharmacokinetics but can suffer from slow and unspecific release of encapsulated drugs. Membrane-active peptides, based on sequences derived or inspired from antimicrobial peptides (AMPs), could offer means to trigger and control the release. Cholesterol is used in most liposomal drug delivery systems (DDS) to improve the stability of the formulation, but the activity of AMPs on cholesterol-rich membranes tends to be very low, complicating peptide-triggered release strategies. Here, we show a de novo designed AMP-mimetic peptide that efficiently triggers content release from cholesterol-containing lipid vesicles when covalently conjugated to headgroup-functionalized lipids. Binding to vesicles induces peptide folding and triggers a lipid phase separation, which in the presence of cholesterol results in high local peptide concentrations at the lipid bilayer surface and rapid content release. We anticipate that these results will facilitate the development of peptide-based strategies for controlling and triggering drug release from liposomal drug delivery systems.
AU  - Utterström,J
AU  - Barriga,HMG
AU  - Holme,MN
AU  - Selegård,R
AU  - Stevens,MM
AU  - Aili,D
DO  - 10.1021/acs.bioconjchem.2c00115
EP  - 746
PY  - 2022///
SN  - 1043-1802
SP  - 736
TI  - Peptide-folding triggered phase separation and lipid membrane destabilization in cholesterol-rich lipid vesicles.
T2  - Bioconjugate Chemistry
UR  - http://dx.doi.org/10.1021/acs.bioconjchem.2c00115
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35362952
UR  - https://pubs.acs.org/doi/10.1021/acs.bioconjchem.2c00115
UR  - http://hdl.handle.net/10044/1/96534
VL  - 33
ER  -
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