Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Najer:2022:10.1002/smll.202201993,
author = {Najer, A and Belessiotis, Richards A and Kim, H and Saunders, C and Adrianus, C and Fenaroli, F and Che, J and Tonkin, R and Hogset, H and Loercher, S and Penna, M and Higgins, S and Meier, W and Yarovsky, I and Stevens, MM},
doi = {10.1002/smll.202201993},
journal = {Small},
title = {Block length-dependent protein fouling on Poly(2-oxazoline)-based polymersomes: influence on macrophage association and circulation behavior},
url = {http://dx.doi.org/10.1002/smll.202201993},
volume = {18},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Polymersomes are vesicular structures self-assembled from amphiphilic block copolymers and are considered an alternative to liposomes for applications in drug delivery, immunotherapy, biosensing, and as nanoreactors and artificial organelles. However, the limited availability of systematic stability, protein fouling (protein corona formation), and blood circulation studies hampers their clinical translation. Poly(2-oxazoline)s (POx) are valuable antifouling hydrophilic polymers that can replace the current gold-standard, poly(ethylene glycol) (PEG), yet investigations of POx functionality on nanoparticles are relatively sparse. Herein, a systematic study is reported of the structural, dynamic and antifouling properties of polymersomes made of poly(2-methyl-2-oxazoline)-block-poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PMOXA-b-PDMS-b-PMOXA). The study relates in vitro antifouling performance of the polymersomes to atomistic molecular dynamics simulations of polymersome membrane hydration behavior. These observations support the experimentally demonstrated benefit of maximizing the length of PMOXA (degree of polymerization (DP) > 6) while keeping PDMS at a minimal length that still provides sufficient membrane stability (DP > 19). In vitro macrophage association and in vivo blood circulation evaluation of polymersomes in zebrafish embryos corroborate these findings. They further suggest that single copolymer presentation on polymersomes is outperformed by blends of varied copolymer lengths. This study helps to rationalize design rules for stable and low-fouling polymersomes for future medical applications.
AU  - Najer,A
AU  - Belessiotis,Richards A
AU  - Kim,H
AU  - Saunders,C
AU  - Adrianus,C
AU  - Fenaroli,F
AU  - Che,J
AU  - Tonkin,R
AU  - Hogset,H
AU  - Loercher,S
AU  - Penna,M
AU  - Higgins,S
AU  - Meier,W
AU  - Yarovsky,I
AU  - Stevens,MM
DO  - 10.1002/smll.202201993
PY  - 2022///
SN  - 1613-6810
TI  - Block length-dependent protein fouling on Poly(2-oxazoline)-based polymersomes: influence on macrophage association and circulation behavior
T2  - Small
UR  - http://dx.doi.org/10.1002/smll.202201993
UR  - https://onlinelibrary.wiley.com/doi/10.1002/smll.202201993
UR  - http://hdl.handle.net/10044/1/97511
VL  - 18
ER  -
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