Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pentinmikko:2022:10.1126/sciadv.abm1847,
author = {Pentinmikko, N and Lozano, R and Scharaw, S and Andersson, S and Englund, JI and Castillo-Azofeifa, D and Gallagher, A and Broberg, M and Song, K-Y and Sola, Carvajal A and Speidel, AT and Sundstrom, M and Allbritton, N and Stevens, MM and Klein, OD and Teixeira, A and Katajisto, P},
doi = {10.1126/sciadv.abm1847},
journal = {Science Advances},
pages = {1--13},
title = {Cellular shape reinforces niche to stem cell signaling in the small intestine.},
url = {http://dx.doi.org/10.1126/sciadv.abm1847},
volume = {8},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Niche-derived factors regulate tissue stem cells, but apart from the mechanosensory pathways, the effect of niche geometry is not well understood. We used organoids and bioengineered tissue culture platforms to demonstrate that the conical shape of Lgr5+ small intestinal stem cells (ISCs) facilitate their self-renewal and function. Inhibition of non-muscle myosin II (NM II)-driven apical constriction altered ISC shape and reduced niche curvature and stem cell capacity. Niche curvature is decreased in aged mice, suggesting that suboptimal interactions between old ISCs and their niche develop with age. We show that activation of NM IIC or physical restriction to young topology improves in vitro regeneration by old epithelium. We propose that the increase in lateral surface area of ISCs induced by apical constriction promotes interactions between neighboring cells, and the curved topology of the intestinal niche has evolved to maximize signaling between ISCs and neighboring cells.
AU  - Pentinmikko,N
AU  - Lozano,R
AU  - Scharaw,S
AU  - Andersson,S
AU  - Englund,JI
AU  - Castillo-Azofeifa,D
AU  - Gallagher,A
AU  - Broberg,M
AU  - Song,K-Y
AU  - Sola,Carvajal A
AU  - Speidel,AT
AU  - Sundstrom,M
AU  - Allbritton,N
AU  - Stevens,MM
AU  - Klein,OD
AU  - Teixeira,A
AU  - Katajisto,P
DO  - 10.1126/sciadv.abm1847
EP  - 13
PY  - 2022///
SN  - 2375-2548
SP  - 1
TI  - Cellular shape reinforces niche to stem cell signaling in the small intestine.
T2  - Science Advances
UR  - http://dx.doi.org/10.1126/sciadv.abm1847
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36240269
UR  - https://www.science.org/doi/10.1126/sciadv.abm1847
UR  - http://hdl.handle.net/10044/1/100574
VL  - 8
ER  -
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