Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{He:2016:10.1002/jbm.b.33668,
author = {He, M and Callanan, A and Lagaras, K and Steele, JA and Stevens, MM},
doi = {10.1002/jbm.b.33668},
journal = {Journal of Biomedical Materials Research Part B: Applied Biomaterials},
pages = {1352--1360},
title = {Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys.},
url = {http://dx.doi.org/10.1002/jbm.b.33668},
volume = {105},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Renal transplantation is well established as the optimal form of renal replacement therapy but is restricted by the limited pool of organs available for transplantation. The whole organ decellularisation approach is leading the way for a regenerative medicine solution towards bioengineered organ replacements. However, systematic preoptimization of both decellularization and recellularization parameters is essential prior to any potential clinical application and should be the next stage in the evolution of whole organ decellularization as a potential strategy for bioengineered organ replacements. Here we have systematically assessed two fundamental parameters (concentration and duration of perfusion) with regards to the effects of differing exposure to the most commonly used single decellularizing agent (sodium dodecyl sulphate/SDS) in the perfusion decellularization process for whole rat kidney ECM bioscaffolds, with findings showing improved preservation of both structural and functional components of the whole kidney ECM bioscaffold. Whole kidney bioscaffolds based on our enhanced protocol were successfully recellularized with rat primary renal cells and mesenchymal stromal cells. These findings should be widely applicable to decellularized whole organ bioscaffolds and their optimization in the development of regenerated organ replacements for transplantation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2016.
AU  - He,M
AU  - Callanan,A
AU  - Lagaras,K
AU  - Steele,JA
AU  - Stevens,MM
DO  - 10.1002/jbm.b.33668
EP  - 1360
PY  - 2016///
SN  - 1552-4973
SP  - 1352
TI  - Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys.
T2  - Journal of Biomedical Materials Research Part B: Applied Biomaterials
UR  - http://dx.doi.org/10.1002/jbm.b.33668
UR  - http://hdl.handle.net/10044/1/45790
VL  - 105
ER  -
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