Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kallepitis:2017:10.1038/ncomms14843,
author = {Kallepitis, C and Bergholt, MS and Mazo, MM and Leonardo, V and Skaalure, SC and Maynard, SA and Stevens, MM},
doi = {10.1038/ncomms14843},
journal = {Nature Communications},
title = {Quantitative volumetric Raman imaging of three dimensional cell cultures},
url = {http://dx.doi.org/10.1038/ncomms14843},
volume = {8},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in 3D cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy.
AU  - Kallepitis,C
AU  - Bergholt,MS
AU  - Mazo,MM
AU  - Leonardo,V
AU  - Skaalure,SC
AU  - Maynard,SA
AU  - Stevens,MM
DO  - 10.1038/ncomms14843
PY  - 2017///
SN  - 2041-1723
TI  - Quantitative volumetric Raman imaging of three dimensional cell cultures
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms14843
UR  - http://hdl.handle.net/10044/1/44038
VL  - 8
ER  -
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