Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kim:2017:10.1002/adma.201701086,
author = {Kim, E and Zwi, Dantsis L and Reznikov, N and Hansel, CS and Agarwal, S and Stevens, MM},
doi = {10.1002/adma.201701086},
journal = {Advanced Materials},
title = {One-pot synthesis of multiple protein-encapsulated DNA flowers and their application in intracellular protein delivery},
url = {http://dx.doi.org/10.1002/adma.201701086},
volume = {29},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Inspired by biological systems, many biomimetic methods suggest fabrication of functional materials with unique physicochemical properties. Such methods frequently generate organic–inorganic composites that feature highly ordered hierarchical structures with intriguing properties, distinct from their individual components. A striking example is that of DNA–inorganic hybrid micro/nanostructures, fabricated by the rolling circle technique. Here, a novel concept for the encapsulation of bioactive proteins in DNA flowers (DNF) while maintaining the activity of protein payloads is reported. A wide range of proteins, including enzymes, can be simultaneously associated with the growing DNA strands and Mg2PPi crystals during the rolling circle process, ultimately leading to the direct immobilization of proteins into DNF. The unique porous structure of this construct, along with the abundance of Mg ions and DNA molecules present, provides many interaction sites for proteins, enabling high loading efficiency and enhanced stability. Further, as a proof of concept, it is demonstrated that the DNF can deliver payloads of cytotoxic protein (i.e., RNase A) to the cells without a loss in its biological function and structural integrity, resulting in highly increased cell death compared to the free protein.
AU  - Kim,E
AU  - Zwi,Dantsis L
AU  - Reznikov,N
AU  - Hansel,CS
AU  - Agarwal,S
AU  - Stevens,MM
DO  - 10.1002/adma.201701086
PY  - 2017///
SN  - 1521-4095
TI  - One-pot synthesis of multiple protein-encapsulated DNA flowers and their application in intracellular protein delivery
T2  - Advanced Materials
UR  - http://dx.doi.org/10.1002/adma.201701086
UR  - http://hdl.handle.net/10044/1/46182
VL  - 29
ER  -
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