Publications
170 results found
Patel BV, Wilson MR, O'Dea KP, et al., 2013, TNF-Induced Death Signaling Triggers Alveolar Epithelial Dysfunction in Acute Lung Injury, J Immunol, Vol: 190, Pages: 4274-4282
Edey LF, O'Dea KP, Herbert BR, et al., 2013, The LPS Preterm Labour Model in Mice: An Inverse Relationship between Leukocyte Recruitment to the Myometrium and Delivery Time., 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 93A-94A, ISSN: 1933-7191
Edey LF, O'Dea KP, Herbert BR, et al., 2013, The Ability of Progesterone To Regulate the Inflammatory Monocyte Subset during Pregnancy, 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 121A-122A, ISSN: 1933-7191
Wilson MR, Takata M, 2013, Inflammatory mechanisms of ventilator-induced lung injury: a time to stop and think?, Anaesthesia, Vol: 68, Pages: 175-178
Huang J, Kumar S, Singanayagam A, et al., 2013, Exhaled breath acetone for therapeutic monitoring in pneumonia using selected ion flow tube mass spectrometry (SIFT-MS), ANALYTICAL METHODS, Vol: 5, Pages: 3807-3810, ISSN: 1759-9660
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- Citations: 11
Tatham KC, Xiao W, O'Dea KP, et al., 2013, Neutrophil-Dependent Shedding Of Heparan Sulfate From The Pulmonary Endothelium In An In Vitro Model Of Simulated Ischemia, American Journal of Respiratory and Critical Care Medicine, Vol: 187
Wakabayashi K, Wilson MR, Patel BV, et al., 2013, Inhibition Of TNF Receptor p55 By A Domain Antibody Attenuates Acid-Induced Lung Injury In Mice, American Thoracic Society
RationaleTumor necrosis factor (TNF) is strongly implicated in the evolution of acute lung injury (ALI), but its potential as a therapeutic target has been hampered by its complex biology. TNF signals through two cell surface receptors, p55 and p75, which play differential roles in ALI, with p55 promoting injury while p75 opposes this. We have recently shown that selective p55 blockade using novel domain antibody technology attenuates ventilator-induced lung injury (VILI) in mice. To further translate these findings to the bedside, we explored the potential for selective blockade of p55 in a clinically relevant mouse model of acid-induced ALI.MethodsMale C57BL6 mice were pretreated with intranasal administration of either specific domain antibody to p55 (dAb) or non-specific control domain antibody (‘dummy’). Four hours later, the mice were either instrumented for analysis without further instillation (i.e. baseline) or intratracheally challenged with hydrochloric acid (0.1M, 75μl) via an oro-tracheal tube to induce ALI. All acid-challenged animals were instrumented for analysis at 24 hours after acid instillation. Analysis consisted of mice being anesthetized, tracheostomized, and mechanically ventilated in order to evaluate PaO2/FiO2 ratio and respiratory mechanics. The left lung was taken for determining wet/dry ratio and a lunglavage sample was taken from the right lung to measure protein levels and neutrophil counts.ResultsHydrochloric acid instillation in the dummy group resulted in severe ALI, represented by hypoxemia, elastance increase, pulmonary edema and inflammation (Table 1). These mice were also visually very sick, showing considerably reduced locomotion and responsiveness. In contrast, pretreatment with p55 dAb dramatically improved the clinical symptoms, along with significant attenuation in all the physiological markers of lung injury. However, the dAb treatment did not significantly attenuate lung neutrophil recruitment represented b
Lax S, Wilson MR, Takata M, et al., 2012, THE ROLE OF PRE-RECEPTOR GLUCOCORTICOID METABOLISM IN REGULATING THE SEVERITY AND PERSISTENCE OF MURINE LUNG INJURY, Winter Meeting of the British-Thoracic-Society 2012, Publisher: BMJ PUBLISHING GROUP, Pages: A28-A28, ISSN: 0040-6376
Blackbeard J, Wallace VC, O'Dea KP, et al., 2012, The correlation between pain-related behaviour and spinal microgliosis in four distinct models of peripheral neuropathy, Eur.J Pain, Vol: 16, Pages: 1357-1367
BACKGROUND: Peripheral nerve injury is associated with a spinal microglial response that has been correlated with the development of behaviours reflective of neuropathic pain. METHODS: To examine whether this phenomenon is generalizable to neuropathic pain of non-traumatic aetiology, this study investigated the association between spinal microgliosis and behavioural measures of neuropathic hypersensitivity and pain-related anxiety behaviour in four distinct rat models of peripheral neuropathic pain. These were traumatic neuropathy [L5 spinal nerve transection (SNT)], HIV-related neuropathies (either treatment with the antiretroviral drug Zalcitabine (ddC) or combination of perineural exposure to the HIV-gp120 protein and ddC treatment) and varicella zoster virus (VZV) infection. RESULTS AND CONCLUSION: Persistent mechanical hypersensitivity developed in all 'neuropathic' rats. However, spinal microgliosis, as measured by increased CD11b/c immunohistochemical staining and increased numbers of cells expressing CD11b measured by flow cytometry, was evident in the SNT and to a lesser extent in the HIV neuropathy models but not the VZV model. These results suggest that behavioural hypersensitivity and thigmotaxis can only be linked to a microglial response in certain models of neuropathy
Palazzo S, Wall C, James E, et al., 2012, SEDATION, VENTILATION ANTI CARDIOVASCULAR SUPPORT IN BURNS INTENSIVE CARE UNIT PATIENTS, Publisher: SPRINGER, Pages: S221-S221, ISSN: 0342-4642
Wilson MR, Patel BV, Takata M, 2012, Ventilation with "clinically relevant" high tidal volumes does not promote stretch-induced injury in the lungs of healthy mice, CRITICAL CARE MEDICINE, Vol: 40, Pages: 2850-2857, ISSN: 0090-3493
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- Citations: 49
Petrie J, Wilson MR, Patel BV, et al., 2012, Obesity induced by high fat feeding attenuates ventilator-induced lung injury in mice., European Respiratory Society Annual Congress
BackgroundRetrospective analysis of Intensive Care data suggests that obesity may confer a survival advantage in Acute Lung Injury (ALI). Development of ventilator-induced lung injury (VILI) is a major determinant of ALI mortality. We have therefore investigated the impact of high fat diet-induced obesity on VILI in mice.MethodsMale C57BL/6 mice were fed high fat diet for a minimum of 13 weeks, resulting in a mean body weight 30% greater than lean age-matched controls. Animals were anaesthetised and ventilated with high-stretch, standardised as plateau pressure (Pplat) 35-37cmH2O. Tidal volumes were similar between groups (mean ∽1080μl). Mice were ventilated for 180mins or until Pplat increased by 20%. Lung tissue was harvested for wet:dry ratio or processed to a single cell suspension for leukocyte quantification by flow cytometry.ResultsHigh stretch ventilation induced increases in Pplat and lung wet:dry ratio, and a decrease in paO2 in lean mice, which were all significantly attenuated in obese animals. Leukocyte recruitment (cells/g dry lung mass) also tended to be reduced. High fat feeding attenuates pulmonary oedema and lung dysfunction associated with VILI in mice. Numerous metabolic and immunological differences exist between lean and obese subjects. Exploring the mechanisms behind this obesity-mediated protection from VILI may lead to identification of novel pathways and therapeutic targets.Funded by: BJA/Royal College of Anaesthetists.
Patel BV, Wilson MR, Takata M, 2012, Resident alveolar macrophages mediate early alveolar epithelial death signaling and dysfunction., European Respiratory Society Annual Congress
Acute lung injury (ALI) is characterized by alveolar epithelial dysfunction. We previously showed that early epithelial dysfunction was specifically mediated through tumor necrosis factor (TNF) p55 receptor signaling [1]. This study examined the contribution of resident alveolar macrophages (AM) to this phenomenon following acid aspiration.C57Bl6 mice were treated intratracheally with liposomes containing either clodronate or PBS. After 48 hours, they underwent intratracheal instillation of hydrochloric acid followed by mechanical ventilation to assess respiratory parameters. Oxygenation, respiratory elastance, alveolar TNF concentration, lung caspase-8 activity and alveolar fluid clearance (AFC) were measured at 90 minutes after acid instillation.Clodronate liposomes produced an 80% depletion of AMs. AM depletion significantly improved the deterioration in respiratory elastance (cmH2O/µl: PBS=0.06±0.008; CLOD=0.05±0.004; p<0.05) and PaO2:FiO2 (PBS=304±113; CLOD=426±41; P<0.05) induced by acid instillation. Additionally, alveolar TNF was significantly reduced (pg/ml: PBS=46.5±25.8; CLOD=15.5±2.7; P<0.05), along with attenuated lung caspase-8 activity (arbitrary units: PBS=14763±5466; CLOD=7135±372; P<0.01), and improved AFC (%/30min: PBS=3.8±2.6; CLOD=7.1±2.4; P<0.05). Caspase-8 activity showed an inverse correlation to AFC (Pearson r=-0.766; P<0.0001) implying epithelial death receptor activation.These data suggests that during ALI induced by acid aspiration, epithelial dysfunction and hypoxemia are a result of epithelial cell death receptor activation by alveolar macrophage-derived TNF.[1] Patel et al. Intensive Care Med. 2011;37(Supplement):S205Supported by Wellcome Trust, UK.
Lax S, Wilson M, Takata M, et al., 2012, The role of pre-receptor glucocorticoid metabolism in regulating the severity of ALI, Publisher: EUROPEAN RESPIRATORY SOC JOURNALS LTD, ISSN: 0903-1936
Zhuang L, Yang T, Zhao H, et al., 2012, The protective profile of argon, helium, and xenon in a model of neonatal asphyxia in rats, CRITICAL CARE MEDICINE, Vol: 40, Pages: 1724-1730, ISSN: 0090-3493
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- Citations: 104
Patel BV, Wilson MR, Takata M, 2012, Resolution of acute lung injury and inflammation – a translational mouse model., European Respiratory Journal, Vol: 39, Pages: 1162-1170
Tavare AN, Perry NJS, Benzonana LL, et al., 2012, Cancer recurrence after surgery: Direct and indirect effects of anesthetic agents*, INTERNATIONAL JOURNAL OF CANCER, Vol: 130, Pages: 1237-1250, ISSN: 0020-7136
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- Citations: 214
Bertok S, Wilson MR, Morley PJ, et al., 2012, Selective inhibition of intra-alveolar p55 TNF receptor attenuates ventilator-induced lung injury, Thorax, Vol: 67, Pages: 244-251
Wilson MR, Patel BV, Takata M, 2011, DO "CLINICALLY RELEVANT" TIDAL VOLUMES REALLY CAUSE VENTILATOR-INDUCED LUNG INJURY IN MICE?, Winter Meeting of the British-Thoracic-Society, Publisher: B M J PUBLISHING GROUP, Pages: A36-A37, ISSN: 0040-6376
O'Dea KP, Dokpesi JO, Tatham KC, et al., 2011, Regulation of monocyte subset proinflammatory responses within the lung microvasculature by the p38 MAPK/MK2 pathway, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 301, Pages: L812-L821, ISSN: 1040-0605
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- Citations: 29
Scott AJ, O'Dea KP, O'Callaghan D, et al., 2011, Reactive Oxygen Species and p38 Mitogen-activated Protein Kinase Mediate Tumor Necrosis Factor α-Converting Enzyme (TACE/ADAM-17) Activation in Primary Human Monocytes, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 286, Pages: 35466-35476
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- Citations: 78
O'Callaghan DJ, O'Dea KP, Gordon AC, et al., 2011, MONOCYTE TACE ACTIVITY PROFILES ARE ALTERED BY INFLAMMATORY STIMULI, INTENSIVE CARE MEDICINE, Vol: 37, Pages: S258-S258, ISSN: 0342-4642
O'Callaghan DJ, O'Dea KP, Gordon AC, et al., 2011, MONOCYTE TACE ACTIVITY MAY RESPOND TO ENVIRONMENTAL STIMULI, INTENSIVE CARE MEDICINE, Vol: 37, Pages: S258-S258, ISSN: 0342-4642
Patel BV, Wilson MR, Takata M, 2011, The p55 TNF receptor promotes alveolar epithelial dysfunction in experimental lung injury., European Society of Intensive Care Medicine
Dorr AD, Wilson MR, Wakabayashi K, et al., 2011, Sources of alveolar soluble TNF receptors during acute lung injury of different etiologies, JOURNAL OF APPLIED PHYSIOLOGY, Vol: 111, Pages: 177-184, ISSN: 8750-7587
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- Citations: 14
Bertok S, Wilson MR, Dorr AD, et al., 2011, Characterization of TNF receptor subtype expression and signaling on pulmonary endothelial cells in mice, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 300, Pages: L781-L789, ISSN: 1040-0605
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- Citations: 22
Wakabayashi K, Wilson MR, O'Dea KP, et al., 2011, Atelectasis Induces Chemokine Upregulation And Lung Injury In The Isolated Perfused Mouse Lung, Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Waite AC, O'Dea KP, Wilson MR, et al., 2010, FLOW CYTOMETRIC DETECTION OF INTRACELLULAR ACTIVATION MARKERS OF PULMONARY CELLS DURING ACUTE LUNG INJURY, Publisher: B M J PUBLISHING GROUP, Pages: A182-A183, ISSN: 0040-6376
Bertok S, Wilson MR, Morley PJ, et al., 2010, BLOCKADE OF INTRAALVEOLAR P55 TNF-RECEPTOR SIGNALLING BY A DOMAIN ANTIBODY DECREASES INFLAMMATION AND OEDEMA IN AN <i>IN VIVO</i> MOUSE MODEL OF VENTILATOR-INDUCED LUNG INJURY, British-Thoracic-Society-Winter-Meeting 2010, Publisher: B M J PUBLISHING GROUP, Pages: A1-A1, ISSN: 0040-6376
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- Citations: 1
Patel BV, Wilson MR, Takata M, 2010, PULMONARY INFILTRATES IN HIV NEGATIVE IMMUNOCOMPROMISED PATIENTS: OUTCOME AND PREDICTIVE FACTORS, THORAX, Vol: 65, Pages: A183-A184, ISSN: 0040-6376
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