Imperial College London
Alternate

ProfessorMasaoTakata

Faculty of Medicine, Department of Surgery & Cancer

Magill Chair in Anaesthetics & Head of Division of APMIC
 
 
 
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Contact

 

+44 (0)20 3315 8816m.takata

 
 
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Location

 

G3.46Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zöllner:2020:10.1097/SHK.0000000000001343,
author = {Zöllner, J and Howe, LG and Edey, LF and O'Dea, KP and Takata, M and Leiper, J and Johnson, MR},
doi = {10.1097/SHK.0000000000001343},
journal = {Shock},
pages = {199--207},
title = {LPS-induced hypotension in pregnancy: the effect of progesterone supplementation},
url = {http://dx.doi.org/10.1097/SHK.0000000000001343},
volume = {53},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Our previous work has shown that pregnancy exacerbates the hypotensive response to both infection and LPS. The high levels of progesterone (P4) associated with pregnancy have been suggested to be responsible for the pregnancy-induced changes in the cardiovascular response to infection. Here, we test the hypothesis that P4 supplementation exacerbates the hypotensive response of the maternal cardiovascular to LPS.Female CD1 mice had radiotelemetry probes implanted to measure haemodynamic function non-invasively and were time-mated. From day 14 of pregnancy, mice received either 10mg of P4 or vehicle alone per day and on day 16, intraperitoneal LPS (10μg of serotype 0111:B4) was injected. In two identically treated cohorts of mice, tissue and serum (for RNA, protein studies) were collected at 6 and 12hours.Administration of LPS resulted in a fall in blood pressure in vehicle treated, but not P4 supplemented mice. This occurred with similar changes in the circulating levels of cytokines, vasoactive factors and in both circulating and tissue inflammatory cell numbers, but with reduced left ventricular expression of cytokines in P4-supplemented mice. However, left ventricular expression of markers of cardiac dysfunction and apoptosis were similar.This study demonstrates that P4 supplementation prevented LPS-induced hypotension in pregnant mice in association with reduced myocardial inflammatory cytokine gene expression. These observations suggest that rather than being detrimental, P4 supplementation has a protective effect on the maternal cardiovascular response to sepsis.
AU  - Zöllner,J
AU  - Howe,LG
AU  - Edey,LF
AU  - O'Dea,KP
AU  - Takata,M
AU  - Leiper,J
AU  - Johnson,MR
DO  - 10.1097/SHK.0000000000001343
EP  - 207
PY  - 2020///
SN  - 1073-2322
SP  - 199
TI  - LPS-induced hypotension in pregnancy: the effect of progesterone supplementation
T2  - Shock
UR  - http://dx.doi.org/10.1097/SHK.0000000000001343
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30998647
UR  - http://hdl.handle.net/10044/1/71285
VL  - 53
ER  -
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