Imperial College London
Alternate

DrMaureenTaylor

Faculty of Natural Sciences, Department of Life Sciences

Reader in Biological Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5281m.taylor

 
 
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Location

 

607Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Taylor:2019:glycob/cwy113,
author = {Taylor, M and Snelling, T and Smith, DF and Drickamer, K},
doi = {glycob/cwy113},
journal = {Glycobiology},
pages = {332--345},
title = {Absence of a human ortholog of rodent Kupffer cell galactose-binding receptor encoded by the CLEC4f gene},
url = {http://dx.doi.org/10.1093/glycob/cwy113},
volume = {29},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The murine CLEC4f gene encodes the Kupffer cell receptor, a galactose-binding receptor containing a C-type carbohydrate-recognition domain. Orthologs have been identified in nearly 100 species. The receptors from rat and mouse have previously been characterized and data presented here show that functional CLEC4f protein is expressed in domestic cattle (Bos taurus). However, the human CLEC4f gene does not encode a functional receptor because a mutation in the splice acceptor site of the final exon prevents appropriate splicing and a missense mutation disrupts the sugar-binding site. Transcriptomic and PCR analysis of transcripts confirms the absence of a spliced transcript containing the final exon and only background levels of transcripts are detected in human tissues. These mutations are also present in the CLEC4f gene in Neanderthals. In contrast to humans, closely related species, including chimpanzees, do have CLEC4f genes that encode full-length receptors. Affinity chromatography and glycan array results demonstrate that the chimpanzee, bovine and murine proteins all bind to galactose, but they show preferences for different subsets of galactose-containing glycans. In non-human primates, the receptor is expressed in spleen rather than in liver. The results indicate that the CLEC4f protein probably has distinct functions in different species. Absence of the receptor precludes using it for targeting of glycoconjugates to cells in human liver. The fact that CLEC4f protein is expressed in spleen in non-human primates and the close evolutionary relationship of the CLEC4f protein to langerin (CD207) suggest that it may function in the immune system, possibly as a pathogen receptor.
AU  - Taylor,M
AU  - Snelling,T
AU  - Smith,DF
AU  - Drickamer,K
DO  - glycob/cwy113
EP  - 345
PY  - 2019///
SN  - 0959-6658
SP  - 332
TI  - Absence of a human ortholog of rodent Kupffer cell galactose-binding receptor encoded by the CLEC4f gene
T2  - Glycobiology
UR  - http://dx.doi.org/10.1093/glycob/cwy113
UR  - http://hdl.handle.net/10044/1/66861
VL  - 29
ER  -
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