Imperial College London
Alternate

Professor Michael Templeton

Faculty of Engineering, Department of Civil and Environmental Engineering

Professor of Public Health Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6099m.templeton

 
 
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Assistant

 

Miss Judith Barritt +44 (0)20 7594 5967

 
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Location

 

303Skempton BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kelwick:2021:10.1016/j.mattod.2021.02.018,
author = {Kelwick, RJR and Webb, AJ and Wang, Y and Heliot, A and Allan, F and Emery, AM and Templeton, MR and Freemont, PS},
doi = {10.1016/j.mattod.2021.02.018},
journal = {Materials Today},
pages = {25--37},
title = {AL-PHA beads: bioplastic-based protease biosensors for global health applications},
url = {http://dx.doi.org/10.1016/j.mattod.2021.02.018},
volume = {47},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Proteases are multi-functional proteolytic enzymes that have complex roles in human health and disease. Therefore, the development of protease biosensors can be beneficial to global health applications. To this end, we developed Advanced proteoLytic detector PolyHydroxyAlkanoates (AL-PHA) beads – a library of over 20 low-cost, biodegradable, bioplastic-based protease biosensors. Broadly, these biosensors utilise PhaC-reporter fusion proteins that are bound to microbially manufactured polyhydroxyalkanoate beads. In the presence of a specific protease, superfolder green fluorescent reporter proteins are cleaved from the AL-PHA beads – resulting in a loss of bead fluorescence. The Tobacco Etch Virus (TEV) AL-PHA biosensor detected the proteolytic activity of at least 1.85pM of AcTEV. AL-PHA beads were also engineered to detect cercarial elastase from Schistosoma mansoni-derived cercarial transformation fluid (SmCTF) samples, as well as cancer-associated metalloproteinases in extracellular vesicle and cell-conditioned media samples. We envision that AL-PHA beads could be further developed for use in resource-limited settings.
AU  - Kelwick,RJR
AU  - Webb,AJ
AU  - Wang,Y
AU  - Heliot,A
AU  - Allan,F
AU  - Emery,AM
AU  - Templeton,MR
AU  - Freemont,PS
DO  - 10.1016/j.mattod.2021.02.018
EP  - 37
PY  - 2021///
SN  - 1369-7021
SP  - 25
TI  - AL-PHA beads: bioplastic-based protease biosensors for global health applications
T2  - Materials Today
UR  - http://dx.doi.org/10.1016/j.mattod.2021.02.018
UR  - https://www.sciencedirect.com/science/article/pii/S1369702121000663
UR  - http://hdl.handle.net/10044/1/87883
VL  - 47
ER  -
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