Imperial College London
Alternate

DrManiccamThavarajah

Faculty of Medicine, Department of Surgery & Cancer

Senior Teaching Fellow in Anatomy
 
 
 
//

Contact

 

+44 (0)20 3311 7321m.thavarajah Website

 
 
//

Location

 

14L06Lab BlockCharing Cross Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Frazer:1994:10.1016/s1063-4584(05)80075-5,
author = {Frazer, A and Bunning, RA and Thavarajah, M and Seid, JM and Russell, RG},
doi = {10.1016/s1063-4584(05)80075-5},
journal = {Osteoarthritis Cartilage},
pages = {235--245},
title = {Studies on type II collagen and aggrecan production in human articular chondrocytes in vitro and effects of transforming growth factor-beta and interleukin-1beta.},
url = {http://dx.doi.org/10.1016/s1063-4584(05)80075-5},
volume = {2},
year = {1994}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Type II collagen and aggrecan are major components of the extracellular matrix of articular cartilage. Their biosynthesis and catabolism are regulated by chondrocytes. They may be used as markers of chondrocyte phenotype for cells cultured in vitro. Type II collagen gene expression was detected by amplification of type II collagen-specific sequences, using cDNA produced by reverse transcription of mRNA extracted from freshly isolated and cultured human articular chondrocytes by the polymerase chain reaction (PCR). The synthesis of gene product was confirmed by immunohistochemical localization of type II collagen in cartilage sections and in cultured chondrocytes. Aggrecan core protein was also immunolocalized in cartilage sections and in chondrocytes in culture. Expression of type II collagen or aggrecan was not detected immunohistochemically in skin or bone. These results demonstrate that human articular chondrocytes can be characterized in culture, by the combined application of PCR and immunohistochemistry. Interleukin-1beta (IL-1beta) may play an important role in the destruction of cartilage matrix in arthritis, whereas transforming growth factor-beta (TGFbeta) may have an opposing effect and their combined actions may modulate chondrocyte phenotype. The effect of rhIL-1beta and rhTGFbeta on the production of type II collagen by chondrocytes in culture was investigated. It was shown that TGFbeta enhanced the production of type II collagen, localized immunocytochemically, in cultured chondrocytes. IL-1beta inhibited expression of mRNA for type II collagen. The implications of this study, in terms of a better understanding of degenerative cartilage disease, are discussed.
AU  - Frazer,A
AU  - Bunning,RA
AU  - Thavarajah,M
AU  - Seid,JM
AU  - Russell,RG
DO  - 10.1016/s1063-4584(05)80075-5
EP  - 245
PY  - 1994///
SN  - 1063-4584
SP  - 235
TI  - Studies on type II collagen and aggrecan production in human articular chondrocytes in vitro and effects of transforming growth factor-beta and interleukin-1beta.
T2  - Osteoarthritis Cartilage
UR  - http://dx.doi.org/10.1016/s1063-4584(05)80075-5
UR  - https://www.ncbi.nlm.nih.gov/pubmed/11550708
VL  - 2
ER  -
Download