Imperial College London
Alternate

DrMichaelThemis

Faculty of Natural Sciences, Department of Life Sciences (Silwood Park)

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)1895 267 252m.themis

 
 
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Location

 

Workspace 15Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Coutelle:2012,
author = {Coutelle, C and Waddington, SN and Themis, M},
pages = {329--340},
title = {Monitoring for potential adverse effects of prenatal gene therapy: mouse models for developmental aberrations and inadvertent germ line transmission.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22648779},
volume = {891},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - So far no systematic studies have been conducted to investigate developmental aberrations after prenatal gene transfer in mice. Here, we suggest procedures for such observations to be applied, tested and improved in further in utero gene therapy experiments. They are based on our own experience in husbandry for transgenic human diseases mouse models and breading, rearing, and observing mice after fetal gene transfer as well as on the systematic screens for monitoring of knock-out mutant mouse phenotypes established in international mutagenesis projects (EUMORPHIA and EUMODIC and subsequently the International Mouse Phenotyping Consortium). We also describe here the analysis procedures for detection of germ line mutations based on quantitative PCR (qPCR) by sperm-DNA analysis and breeding studies.
AU  - Coutelle,C
AU  - Waddington,SN
AU  - Themis,M
EP  - 340
PY  - 2012///
SP  - 329
TI  - Monitoring for potential adverse effects of prenatal gene therapy: mouse models for developmental aberrations and inadvertent germ line transmission.
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22648779
VL  - 891
ER  -
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