Publications
89 results found
Vázquez-Fernández E, Vos MR, Afanasyev P, et al., 2016, The Structural Architecture of an Infectious Mammalian Prion Using Electron Cryomicroscopy, PLOS Pathogens, Vol: 12, ISSN: 1553-7366
The structure of the infectious prion protein (PrPSc), which is responsible for Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy, has escaped all attempts at elucidation due to its insolubility and propensity to aggregate. PrPSc replicates by converting the non-infectious, cellular prion protein (PrPC) into the misfolded, infectious conformer through an unknown mechanism. PrPSc and its N-terminally truncated variant, PrP 27-30, aggregate into amorphous aggregates, 2D crystals, and amyloid fibrils. The structure of these infectious conformers is essential to understanding prion replication and the development of structure-based therapeutic interventions. Here we used the repetitive organization inherent to GPI-anchorless PrP 27-30 amyloid fibrils to analyze their structure via electron cryomicroscopy. Fourier-transform analyses of averaged fibril segments indicate a repeating unit of 19.1 Å. 3D reconstructions of these fibrils revealed two distinct protofilaments, and, together with a molecular volume of 18,990 Å3, predicted the height of each PrP 27-30 molecule as ~17.7 Å. Together, the data indicate a four-rung β-solenoid structure as a key feature for the architecture of infectious mammalian prions. Furthermore, they allow to formulate a molecular mechanism for the replication of prions. Knowledge of the prion structure will provide important insights into the self-propagation mechanisms of protein misfolding.
Afanasyev P, Ravelli RBG, Matadeen R, et al., 2015, A posteriori correction of camera characteristics from large image data sets, Scientific Reports, Vol: 5, ISSN: 2045-2322
Large datasets are emerging in many fields of image processing including: electron microscopy, light microscopy, medical X-ray imaging, astronomy, etc. Novel computer-controlled instrumentation facilitates the collection of very large datasets containing thousands of individual digital images. In single-particle cryogenic electron microscopy (“cryo-EM”), for example, large datasets are required for achieving quasi-atomic resolution structures of biological complexes. Based on the collected data alone, large datasets allow us to precisely determine the statistical properties of the imaging sensor on a pixel-by-pixel basis, independent of any “a priori” normalization routinely applied to the raw image data during collection (“flat field correction”). Our straightforward “a posteriori” correction yields clean linear images as can be verified by Fourier Ring Correlation (FRC), illustrating the statistical independence of the corrected images over all spatial frequencies. The image sensor characteristics can also be measured continuously and used for correcting upcoming images.
van Heel M, 2013, Finding trimeric HIV-1 envelope glycoproteins in random noise, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: E4175-E4177, ISSN: 0027-8424
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- Citations: 48
Bebeacua C, Tremblay D, Farenc C, et al., 2013, Structure, Adsorption to Host, and Infection Mechanism of Virulent Lactococcal Phage p2, JOURNAL OF VIROLOGY, Vol: 87, Pages: 12302-12312, ISSN: 0022-538X
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- Citations: 70
Nederlof I, Li YW, van Heel M, et al., 2013, Imaging protein three-dimensional nanocrystals with cryo-EM, ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Vol: 69, Pages: 852-859, ISSN: 2059-7983
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- Citations: 31
Manzine LR, Balasco Serrao VH, Trambaioli da Rocha e Lima LM, et al., 2013, Assembly stoichiometry of bacterial selenocysteine synthase and SelC (tRNA<SUP>sec</SUP>), FEBS LETTERS, Vol: 587, Pages: 906-911, ISSN: 0014-5793
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- Citations: 7
Bebeacua C, Lai L, Vegge CS, et al., 2013, Visualizing a Complete <i>Siphoviridae</i> Member by Single-Particle Electron Microscopy: the Structure of Lactococcal Phage TP901-1, JOURNAL OF VIROLOGY, Vol: 87, Pages: 1061-1068, ISSN: 0022-538X
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- Citations: 49
Bebeacua C, Bron P, Lai L, et al., 2010, Structure and Molecular Assignment of Lactococcal Phage TP901-1 Baseplate, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 39079-39086, ISSN: 0021-9258
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- Citations: 49
Sciara G, Bebeacua C, Bron P, et al., 2010, Structure of lactococcal phage p2 baseplate and its mechanism of activation, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 6852-6857, ISSN: 0027-8424
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- Citations: 122
Baier J, Gabrielsen M, Oellerich S, et al., 2009, Spectral Diffusion and Electron-Phonon Coupling of the B800 BChl a Molecules in LH2 Complexes from Three Different Species of Purple Bacteria, BIOPHYSICAL JOURNAL, Vol: 97, Pages: 2604-2612, ISSN: 0006-3495
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- Citations: 22
Marles-Wright J, Grant T, Delumeau O, et al., 2008, Molecular architecture of the "stressosome," a signal integration and transduction hub, SCIENCE, Vol: 322, Pages: 92-96, ISSN: 0036-8075
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- Citations: 125
Hildner R, Lemmer U, Scherf U, et al., 2007, Revealing the electron-phonon coupling in a conjugated polymer by single-molecule spectroscopy, ADVANCED MATERIALS, Vol: 19, Pages: 1978-+, ISSN: 0935-9648
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- Citations: 32
Costa A, Pape T, van Heel M, et al., 2006, Structural basis of the <i>Methanothermobacter thermautotrophicus</i> MCM helicase activity, NUCLEIC ACIDS RESEARCH, Vol: 34, Pages: 5829-5838, ISSN: 0305-1048
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- Citations: 53
Costa A, Pape T, van Heel M, et al., 2006, Structural studies of the archaeal MCM complex in different functional states, JOURNAL OF STRUCTURAL BIOLOGY, Vol: 156, Pages: 210-219, ISSN: 1047-8477
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- Citations: 44
Ray P, Hall RJ, Finn RD, et al., 2005, Conformational changes of <i>Escherichia coli</i> σ<SUP>54</SUP>-RNA-polymerase upon closed-promoter complex formation, JOURNAL OF MOLECULAR BIOLOGY, Vol: 354, Pages: 201-205, ISSN: 0022-2836
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- Citations: 4
van Duinen G, van Heel M, Patwardhan A, 2005, Magnification variations due to illumination curvature and object defocus in transmission electron microscopy, OPTICS EXPRESS, Vol: 13, Pages: 9085-9093, ISSN: 1094-4087
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- Citations: 7
van Heel M, Schatz M, 2005, Fourier shell correlation threshold criteria, JOURNAL OF STRUCTURAL BIOLOGY, Vol: 151, Pages: 250-262, ISSN: 1047-8477
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- Citations: 551
Hofmann C, Michel H, van Heel M, et al., 2005, Multivariate analysis of single-molecule spectra:: Surpassing spectral diffusion -: art. no. 195501, PHYSICAL REVIEW LETTERS, Vol: 94, ISSN: 0031-9007
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- Citations: 52
Klaholz BP, Myasnikov AG, van Heel M, 2004, Visualization of release factor 3 on the ribosome during termination of protein synthesis, NATURE, Vol: 427, Pages: 862-865, ISSN: 0028-0836
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- Citations: 108
Plaisier JR, Koning RI, Koerten HK, et al., 2004, TYSON: Robust searching, sorting, and selecting of single particles in electron micrographs, JOURNAL OF STRUCTURAL BIOLOGY, Vol: 145, Pages: 76-83, ISSN: 1047-8477
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- Citations: 14
Pape T, Meka H, Chen SX, et al., 2003, Hexameric ring structure of the full-length archaeal MCM protein complex, EMBO REPORTS, Vol: 4, Pages: 1079-1083, ISSN: 1469-221X
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- Citations: 108
Orlova EV, Gowen B, Dröge A, et al., 2003, Structure of a viral DNA gatekeeper at 10 Å resolution by cryo-electron microscopy, EMBO JOURNAL, Vol: 22, Pages: 1255-1262, ISSN: 0261-4189
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- Citations: 111
Orlova EV, Papakosta M, Booy FP, et al., 2003, Voltage-gated K<SUP>+</SUP> channel from mammalian brain:: 3D structure at 18 Åof the complete (α)<sub>4</sub>(β)<sub>4</sub> complex, JOURNAL OF MOLECULAR BIOLOGY, Vol: 326, Pages: 1005-1012, ISSN: 0022-2836
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- Citations: 37
Klaholz BP, Pape T, Zavialov AV, et al., 2003, Structure of the <i>Escherichia coli</i> ribosomal termination complex with release factor 2, NATURE, Vol: 421, Pages: 90-94, ISSN: 0028-0836
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- Citations: 168
Ray P, Klaholz BP, Finn RD, et al., 2003, Determination of Escherichia coli RNA polymerase structure by single particle cryoelectron microscopy., Methods Enzymol, Vol: 370, Pages: 24-42, ISSN: 0076-6879
Ray P, Klaholz BP, Finn RD, et al., 2003, Determination of <i>Escherichia coli</i> RNA polymerase structure by single particle cryoelectron microscopy, RNA POLYMERASES AND ASSOCIATED FACTORS, PT C, Vol: 370, Pages: 24-+, ISSN: 0076-6879
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- Citations: 4
Stark H, Rodnina MV, Wieden HJ, et al., 2002, Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex, NATURE STRUCTURAL BIOLOGY, Vol: 9, Pages: 849-854, ISSN: 1072-8368
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- Citations: 169
Finn RD, Orlova EV, van Heel M, et al., 2002, Structures of multisubunit DNA-dependent RNA polymerases, 61st Symposium of the Society-for-General-Microbiology, Publisher: CAMBRIDGE UNIV PRESS, Pages: 73-103, ISSN: 0081-1394
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- Citations: 1
Lurz R, Orlova EV, Günther D, et al., 2001, Structural organisation of the head-to-tail interface of a bacterial virus, JOURNAL OF MOLECULAR BIOLOGY, Vol: 310, Pages: 1027-1037, ISSN: 0022-2836
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- Citations: 77
Matadeen R, Sergiev P, Leonov A, et al., 2001, Direct localization by cryo-electron microscopy of secondary structural elements in <i>Escherichia coli</i> 23 S rRNA which differ from the corresponding regions in <i>Haloarcula marismortui</i>, JOURNAL OF MOLECULAR BIOLOGY, Vol: 307, Pages: 1341-1349, ISSN: 0022-2836
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- Citations: 10
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