Imperial College London
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Dr Marcela P. Vizcaychipi

Faculty of Medicine, Department of Surgery & Cancer

Honorary Clinical Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 3315 8903m.vizcaychipi Website

 
 
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Location

 

3.21Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cuddihy:2021:10.1038/s41598-021-00644-5,
author = {Cuddihy, J and Wu, G and Ho, L and Kudo, H and Dannhorn, A and Mandalia, S and Collins, D and Weir, J and Spencer, A and Vizcaychipi, M and Takats, Z and Nagy, I},
doi = {10.1038/s41598-021-00644-5},
journal = {Scientific Reports},
pages = {1--12},
title = {Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin},
url = {http://dx.doi.org/10.1038/s41598-021-00644-5},
volume = {11},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Burn injuries constitute one of the most serious accidental injuries. Increased metabolic rate is a hallmark feature of burn injury. Visualising lactate dehydrogenase (LDH) activity has been previously used to identify metabolic activity differences, hence cell viability and burn depth in burn skin. LDH activity was visualised in injured and uninjured skin from 38 sub-acute burn patients. LDH activity aided the identification of spatially correlating immunocompetent cells in a sub-group of six patients. Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) was used to describe relative lactate and pyruvate abundance in burned and uninjured tissue. LDH activity was significantly increased in the middle and deep regions of burnt skin compared with superficial areas in burnt skin and uninjured tissue and positively correlated with post-burn time. Regions of increased LDH activity showed high pyruvate and low lactate abundance when examined with DESI-MSI. Areas of increased LDH activity exhibited cellular infiltration, including CD3 + and CD4 + T-lymphocytes and CD68 + macrophages. Our data demonstrate a steady increase in functional LDH activity in sub-acute burn wounds linked to cellular infiltration. The cell types associated are related to tissue restructuring and inflammation. This region in burn wounds is likely the focus of dysregulated inflammation and hypermetabolism.
AU  - Cuddihy,J
AU  - Wu,G
AU  - Ho,L
AU  - Kudo,H
AU  - Dannhorn,A
AU  - Mandalia,S
AU  - Collins,D
AU  - Weir,J
AU  - Spencer,A
AU  - Vizcaychipi,M
AU  - Takats,Z
AU  - Nagy,I
DO  - 10.1038/s41598-021-00644-5
EP  - 12
PY  - 2021///
SN  - 2045-2322
SP  - 1
TI  - Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-021-00644-5
UR  - https://www.nature.com/articles/s41598-021-00644-5
UR  - http://hdl.handle.net/10044/1/92656
VL  - 11
ER  -
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