Imperial College London
Alternate

DrMarcoPurbhoo

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 9024marco.purbhoo Website

 
 
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Location

 

QEQM 1010Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Publication Type
Year
to

53 results found

Naiyer MM, Cassidy SA, Magri A, Cowton V, Chen K, Mansour S, Kranidioti H, Mbirbindi B, Rettman P, Harris S, Fanning LJ, Mulder A, Claas FHJ, Davidson AD, Patel AH, Purbhoo MA, Khakoo SIet al., 2017, KIR2DS2 recognizes conserved peptides derived from viral helicases in the context of HLA-C, SCIENCE IMMUNOLOGY, Vol: 2, ISSN: 2470-9468

Journal article

Jamil KM, Hydes TJ, Cheent KS, Cassidy SA, Traherne JA, Jayaraman J, Trowsdale J, Alexander GJ, Little A-M, McFarlane H, Heneghan MA, Purbhoo MA, Khakoo SIet al., 2017, STAT4-associated natural killer cell tolerance following liver transplantation, GUT, Vol: 66, Pages: 352-+, ISSN: 0017-5749

Journal article

Horlock C, Skulte A, Mitra A, Stansfield A, Bhandari S, Ip W, Qasim W, Lowdell MW, Patel S, Friedetzky A, Purbhoo MA, Newton Ket al., 2016, Manufacture of GMP-compliant functional adenovirus-specific T-cell therapy for treatment of post-transplant infectious complications, CYTOTHERAPY, Vol: 18, Pages: 1209-1218, ISSN: 1465-3249

Journal article

Kwakwa K, Savell A, Davies T, Munro I, Parrinello S, Purbhoo MA, Dunsby C, Neil MAA, French PMWet al., 2016, easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy, Journal of Biophotonics, Vol: 9, Pages: 948-957, ISSN: 1864-0648

TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and TIRF taking advantage of multimode optical fibres and multimode diode lasers to provide the required excitation light. Combined with open source software and relatively simple protocols to prepare samples for STORM, including the use of Vectashield for non-TIRF imaging, this approach enables TIRF and STORM imaging of cells labelled with appropriate dyes or expressing suitable fluorescent proteins to become widely accessible at low cost.

Journal article

Aletrari M-O, Sabaratnam K, Zhang R, Dyball S, Seyedmohammad S, Purbhoo MAet al., 2016, Is differential T cell receptor patterning at the immune synapse mediated by glycosylation of the viral ligand UL18 and the inhibitory receptor ILT2?, Spring Meeting on Clinician Scientists in Training, Publisher: ELSEVIER SCIENCE INC, Pages: 18-18, ISSN: 0140-6736

Conference paper

Cassidy S, Naiyer M, Liu M, McCormick C, Patel A, Purbhoo M, Khakoo Set al., 2014, An activating NK cell receptor with specificity for hepatitis C virus, IMMUNOLOGY, Vol: 143, Pages: 93-93, ISSN: 0019-2805

Journal article

Aletrari M-O, Purbhoo MA, 2014, Glycosylation of the HCMV MHC homologue UL18 alters recruitment of the TCR to the immune synapse, IMMUNOLOGY, Vol: 143, Pages: 119-119, ISSN: 0019-2805

Journal article

Cassidy S, Mukherjee S, Myint TM, Mbiribindi B, North H, Traherne J, Mulder A, Claas FHJ, Purbhoo MA, Das J, Khakoo SIet al., 2014, Peptide selectivity discriminates NK cells from KIR2DL2-and KIR2DL3-positive individuals, European Journal of Immunology, Vol: 45, Pages: 492-500, ISSN: 1521-4141

Natural killer cells are controlled by peptide selective inhibitory receptors for MHC class I, including the killer cell immunoglobulin-like receptors (KIRs). Despite having similar ligands, KIR2DL2 and KIR2DL3 confer different levels of protection to infectious disease. To investigate how changes in peptide repertoire may differentially affect NK cell reactivity, NK cells from KIR2DL2 and KIR2DL3 homozygous donors were tested for activity against different combinations of strong inhibitory (VAPWNSFAL), weak inhibitory (VAPWNSRAL), and antagonist peptide (VAPWNSDAL). KIR2DL3-positive NK cells were more sensitive to changes in the peptide content of MHC class I than KIR2DL2-positive NK cells. These differences were observed for the weakly inhibitory peptide VAPWNSRAL in single peptide and double peptide experiments (p < 0.01 and p < 0.03, respectively). More significant differences were observed in experiments using all three peptides (p < 0.0001). Mathematical modeling of the experimental data demonstrated that VAPWNSRAL was dominant over VAPWNSFAL in distinguishing KIR2DL3- from KIR2DL2-positive donors. Donors with different KIR genotypes have different responses to changes in the peptide bound by MHC class I. Differences in the response to the peptide content of MHC class I may be one mechanism underlying the protective effects of different KIR genes against infectious disease.

Journal article

Stanton RJ, Prod'homme V, Purbhoo MA, Moore M, Aicheler RJ, Heinzmann M, Bailer SM, Haas J, Antrobus R, Weekes MP, Lehner PJ, Vojtesek B, Miners KL, Man S, Wilkie GS, Davison AJ, Wang ECY, Tomasec P, Wilkinson GWGet al., 2014, HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells, Cell Host & Microbe, Vol: 16, Pages: 201-214, ISSN: 1934-6069

Immune evasion genes help human cytomegalovirus (HCMV) establish lifelong persistence. Without immune pressure, laboratory-adapted HCMV strains have undergone genetic alterations. Among these, the deletion of the UL/b’ domain is associated with loss of virulence. In a screen of UL/b’, we identified pUL135 as a protein responsible for the characteristic cytopathic effect of clinical HCMV strains that also protected from natural killer (NK) and T cell attack. pUL135 interacted directly with abl interactor 1 (ABI1) and ABI2 to recruit the WAVE2 regulatory complex to the plasma membrane, remodel the actin cytoskeleton and dramatically reduce the efficiency of immune synapse (IS) formation. An intimate association between F-actin filaments in target cells and the IS was dispelled by pUL135 expression. Thus, F-actin in target cells plays a critical role in synaptogenesis, and this can be exploited by pathogens to protect against cytotoxic immune effector cells. An independent interaction between pUL135 and talin disrupted cell contacts with the extracellular matrix.

Journal article

Kumar N, Mroz A, Aletrari M, Goldin R, Purbhoo Met al., 2014, LLT1 IS UPREGULATED IN HEPATOCELLULAR CARCINOMA AND INHIBITS NK CELL CYTOTOXICITY, Annual General Meeting of the British-Society-of-Gastroenterology (BSG), Publisher: BMJ PUBLISHING GROUP, Pages: A92-A92, ISSN: 0017-5749

Conference paper

Satkunanathan S, Kumar N, Bajorek M, Purbhoo MA, Culley FJet al., 2014, Respiratory Syncytial Virus Infection, TLR3 Ligands, and Proinflammatory Cytokines Induce CD161 Ligand LLT1 Expression on the Respiratory Epithelium, JOURNAL OF VIROLOGY, Vol: 88, Pages: 2366-2373, ISSN: 0022-538X

Journal article

Cassidy SA, Liu M, Naiyer MM, Patel AH, Purbhoo M, Khakoo SIet al., 2014, HCV specificity of the activating NK cell receptor KIR2DS2, 65th Annual Meeting of the American-Association-for-the-Study-of-Liver-Diseases, Publisher: WILEY-BLACKWELL, Pages: 1058A-1058A, ISSN: 0270-9139

Conference paper

Cheent KS, Jamil KM, Cassidy S, Liu M, Mbiribindi B, Mulder A, Claas FHJ, Purbhoo MA, Khakoo SIet al., 2013, Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 16981-16986, ISSN: 0027-8424

Journal article

Cheent KS, Jamil KM, Purbhoo M, Khakoo SIet al., 2013, The presence of signal peptides binding HLA-C is associated with spontaneous resolution of HCV infection, 64th Annual Meeting and Postgraduate Course of the American-Association-for-the-Study-of-Liver-Diseases, Publisher: WILEY-BLACKWELL, Pages: 1171A-1172A, ISSN: 0270-9139

Conference paper

Jamil KM, Cheent KS, Heneghan MA, Purbhoo M, Khakoo SIet al., 2013, Gene expression analysis of recipient NK cells after Liver Transplantation demonstrates downregulation of STAT4, 64th Annual Meeting and Postgraduate Course of the American-Association-for-the-Study-of-Liver-Diseases, Publisher: WILEY-BLACKWELL, Pages: 410A-410A, ISSN: 0270-9139

Conference paper

Borhis G, Ahmed PS, Mbiribindi B, Naiyer MM, Davis DM, Purbhoo MA, Khakoo SIet al., 2013, A Peptide Antagonist Disrupts NK Cell Inhibitory Synapse Formation, JOURNAL OF IMMUNOLOGY, Vol: 190, Pages: 2924-2930, ISSN: 0022-1767

Journal article

Purbhoo MA, 2013, The function of sub-synaptic vesicles during T-cell activation, IMMUNOLOGICAL REVIEWS, Vol: 251, Pages: 36-48, ISSN: 0105-2896

Journal article

Jamil KM, Alexander GJ, Cheent KS, Agarwal K, Purbhoo M, Heneghan MA, Khakoo SIet al., 2012, Hepatitis C virus infection abrogates the effect of liver transplantation on NK cell activation, 63rd Annual Meeting of the American-Association-for-the-Study-of-Liver-Diseases (AASLD), Publisher: WILEY-BLACKWELL, Pages: 1107A-1107A, ISSN: 0270-9139

Conference paper

Cheent KS, Jamil KM, Purbhoo M, Khakoo SIet al., 2012, The HLA-G signal peptide antagonises NK cell inhibition due to HCV core35-44 at HLA-C, 63rd Annual Meeting of the American-Association-for-the-Study-of-Liver-Diseases (AASLD), Publisher: WILEY-BLACKWELL, Pages: 1103A-1103A, ISSN: 0270-9139

Conference paper

Purbhoo M, Kumar N, Ashraf S, 2012, LLT1 engages CD161 at the immunological synapse between T cells and hepatocytes, European Congress of Immunology, Publisher: WILEY-BLACKWELL, Pages: 504-504, ISSN: 0019-2805

Conference paper

Aletrari M, Sabaratnam K, Dyball S, Seyedmohammad S, Purbhoo Met al., 2012, The role of inhibitory receptor affinity in differential partitioning of TCR at the immune synapse, European Congress of Immunology, Publisher: WILEY-BLACKWELL, Pages: 155-156, ISSN: 0019-2805

Conference paper

Purbhoo MA, Kumar N, Ashraf S, 2012, LLT1 ENGAGES CD161 AT THE IMMUNOLOGICAL SYNAPSE BETWEEN T CELLS AND HEPATOCYTES, 47th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL), Publisher: ELSEVIER SCIENCE BV, Pages: S315-S315, ISSN: 0168-8278

Conference paper

Sabaratnam K, Dyball S, Seyedmohammad S, Purbhoo MAet al., 2011, Viral ligands reposition activating and inhibitory receptors at the T-cell immune synapse to enhance inhibitory signalling: a novel mechanism for immune escape, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 48-48, ISSN: 0019-2805

Conference paper

Purbhoo MA, Liu H, Oddos S, Owen DM, Neil MAA, Pageon SV, French PMW, Rudd CE, Davis DMet al., 2010, Dynamics of sub-synaptic vesicles and surface microclusters at the T cell immunological synapse, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 175-175, ISSN: 0019-2805

Conference paper

Borhis G, Purbhoo M, Ahmed PS, Fadda L, Davis DM, Khakoo SIet al., 2010, Effects of MHC class I peptides on KIR2DL3 clustering and signalling, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 134-134, ISSN: 0019-2805

Conference paper

Liu H, Purbhoo MA, Davis DM, Rudd CEet al., 2010, SH2 domain containing leukocyte phosphoprotein of 76-kDa (SLP-76) feedback regulation of ZAP-70 microclustering, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 10166-10171, ISSN: 0027-8424

Journal article

Fadda L, Borhis G, Ahmed P, Cheent K, Pageon SV, Cazaly A, Stathopoulos S, Middleton D, Mulder A, Claas FHJ, Elliott T, Davis DM, Purbhoo MA, Khakoo SIet al., 2010, Peptide antagonism as a mechanism for NK cell activation, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 10160-10165, ISSN: 0027-8424

Journal article

Purbhoo MA, Liu H, Oddos S, Owen DM, Neil MAA, Pageon SV, French PMW, Rudd CE, Davis DMet al., 2010, Dynamics of Subsynaptic Vesicles and Surface Microclusters at the Immunological Synapse, SCIENCE SIGNALING, Vol: 3, ISSN: 1945-0877

Journal article

Varela-Rohena A, Molloy PE, Dunn SM, Li Y, Suhoski MM, Carroll RG, Milicic A, Mahon T, Sutton DH, Laugel B, Moysey R, Cameron BJ, Vuidepot A, Purbhoo MA, Cole DK, Phillips RE, June CH, Jakobsen BK, Sewell AK, Riley JLet al., 2008, Control of HIV-1 immune escape by CD8 T cells expressing enhanced T-cell receptor, NATURE MEDICINE, Vol: 14, Pages: 1390-1395, ISSN: 1078-8956

Journal article

Oddos S, Dunsby C, Purbhoo MA, Chauveau A, Owen DM, Neil MAA, Davis DM, French PMWet al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495

Journal article

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