Publications
53 results found
Naiyer MM, Cassidy SA, Magri A, et al., 2017, KIR2DS2 recognizes conserved peptides derived from viral helicases in the context of HLA-C, SCIENCE IMMUNOLOGY, Vol: 2, ISSN: 2470-9468
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- Citations: 59
Jamil KM, Hydes TJ, Cheent KS, et al., 2017, STAT4-associated natural killer cell tolerance following liver transplantation, GUT, Vol: 66, Pages: 352-+, ISSN: 0017-5749
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- Citations: 17
Horlock C, Skulte A, Mitra A, et al., 2016, Manufacture of GMP-compliant functional adenovirus-specific T-cell therapy for treatment of post-transplant infectious complications, CYTOTHERAPY, Vol: 18, Pages: 1209-1218, ISSN: 1465-3249
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- Citations: 10
Kwakwa K, Savell A, Davies T, et al., 2016, easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy, Journal of Biophotonics, Vol: 9, Pages: 948-957, ISSN: 1864-0648
TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and TIRF taking advantage of multimode optical fibres and multimode diode lasers to provide the required excitation light. Combined with open source software and relatively simple protocols to prepare samples for STORM, including the use of Vectashield for non-TIRF imaging, this approach enables TIRF and STORM imaging of cells labelled with appropriate dyes or expressing suitable fluorescent proteins to become widely accessible at low cost.
Aletrari M-O, Sabaratnam K, Zhang R, et al., 2016, Is differential T cell receptor patterning at the immune synapse mediated by glycosylation of the viral ligand UL18 and the inhibitory receptor ILT2?, Spring Meeting on Clinician Scientists in Training, Publisher: ELSEVIER SCIENCE INC, Pages: 18-18, ISSN: 0140-6736
Cassidy S, Naiyer M, Liu M, et al., 2014, An activating NK cell receptor with specificity for hepatitis C virus, IMMUNOLOGY, Vol: 143, Pages: 93-93, ISSN: 0019-2805
Aletrari M-O, Purbhoo MA, 2014, Glycosylation of the HCMV MHC homologue UL18 alters recruitment of the TCR to the immune synapse, IMMUNOLOGY, Vol: 143, Pages: 119-119, ISSN: 0019-2805
Cassidy S, Mukherjee S, Myint TM, et al., 2014, Peptide selectivity discriminates NK cells from KIR2DL2-and KIR2DL3-positive individuals, European Journal of Immunology, Vol: 45, Pages: 492-500, ISSN: 1521-4141
Natural killer cells are controlled by peptide selective inhibitory receptors for MHC class I, including the killer cell immunoglobulin-like receptors (KIRs). Despite having similar ligands, KIR2DL2 and KIR2DL3 confer different levels of protection to infectious disease. To investigate how changes in peptide repertoire may differentially affect NK cell reactivity, NK cells from KIR2DL2 and KIR2DL3 homozygous donors were tested for activity against different combinations of strong inhibitory (VAPWNSFAL), weak inhibitory (VAPWNSRAL), and antagonist peptide (VAPWNSDAL). KIR2DL3-positive NK cells were more sensitive to changes in the peptide content of MHC class I than KIR2DL2-positive NK cells. These differences were observed for the weakly inhibitory peptide VAPWNSRAL in single peptide and double peptide experiments (p < 0.01 and p < 0.03, respectively). More significant differences were observed in experiments using all three peptides (p < 0.0001). Mathematical modeling of the experimental data demonstrated that VAPWNSRAL was dominant over VAPWNSFAL in distinguishing KIR2DL3- from KIR2DL2-positive donors. Donors with different KIR genotypes have different responses to changes in the peptide bound by MHC class I. Differences in the response to the peptide content of MHC class I may be one mechanism underlying the protective effects of different KIR genes against infectious disease.
Stanton RJ, Prod'homme V, Purbhoo MA, et al., 2014, HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells, Cell Host & Microbe, Vol: 16, Pages: 201-214, ISSN: 1934-6069
Immune evasion genes help human cytomegalovirus (HCMV) establish lifelong persistence. Without immune pressure, laboratory-adapted HCMV strains have undergone genetic alterations. Among these, the deletion of the UL/b’ domain is associated with loss of virulence. In a screen of UL/b’, we identified pUL135 as a protein responsible for the characteristic cytopathic effect of clinical HCMV strains that also protected from natural killer (NK) and T cell attack. pUL135 interacted directly with abl interactor 1 (ABI1) and ABI2 to recruit the WAVE2 regulatory complex to the plasma membrane, remodel the actin cytoskeleton and dramatically reduce the efficiency of immune synapse (IS) formation. An intimate association between F-actin filaments in target cells and the IS was dispelled by pUL135 expression. Thus, F-actin in target cells plays a critical role in synaptogenesis, and this can be exploited by pathogens to protect against cytotoxic immune effector cells. An independent interaction between pUL135 and talin disrupted cell contacts with the extracellular matrix.
Kumar N, Mroz A, Aletrari M, et al., 2014, LLT1 IS UPREGULATED IN HEPATOCELLULAR CARCINOMA AND INHIBITS NK CELL CYTOTOXICITY, Annual General Meeting of the British-Society-of-Gastroenterology (BSG), Publisher: BMJ PUBLISHING GROUP, Pages: A92-A92, ISSN: 0017-5749
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- Citations: 1
Satkunanathan S, Kumar N, Bajorek M, et al., 2014, Respiratory Syncytial Virus Infection, TLR3 Ligands, and Proinflammatory Cytokines Induce CD161 Ligand LLT1 Expression on the Respiratory Epithelium, JOURNAL OF VIROLOGY, Vol: 88, Pages: 2366-2373, ISSN: 0022-538X
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- Citations: 29
Cassidy SA, Liu M, Naiyer MM, et al., 2014, HCV specificity of the activating NK cell receptor KIR2DS2, 65th Annual Meeting of the American-Association-for-the-Study-of-Liver-Diseases, Publisher: WILEY-BLACKWELL, Pages: 1058A-1058A, ISSN: 0270-9139
Cheent KS, Jamil KM, Cassidy S, et al., 2013, Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 16981-16986, ISSN: 0027-8424
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- Citations: 22
Cheent KS, Jamil KM, Purbhoo M, et al., 2013, The presence of signal peptides binding HLA-C is associated with spontaneous resolution of HCV infection, 64th Annual Meeting and Postgraduate Course of the American-Association-for-the-Study-of-Liver-Diseases, Publisher: WILEY-BLACKWELL, Pages: 1171A-1172A, ISSN: 0270-9139
Jamil KM, Cheent KS, Heneghan MA, et al., 2013, Gene expression analysis of recipient NK cells after Liver Transplantation demonstrates downregulation of STAT4, 64th Annual Meeting and Postgraduate Course of the American-Association-for-the-Study-of-Liver-Diseases, Publisher: WILEY-BLACKWELL, Pages: 410A-410A, ISSN: 0270-9139
Borhis G, Ahmed PS, Mbiribindi B, et al., 2013, A Peptide Antagonist Disrupts NK Cell Inhibitory Synapse Formation, JOURNAL OF IMMUNOLOGY, Vol: 190, Pages: 2924-2930, ISSN: 0022-1767
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- Citations: 23
Purbhoo MA, 2013, The function of sub-synaptic vesicles during T-cell activation, IMMUNOLOGICAL REVIEWS, Vol: 251, Pages: 36-48, ISSN: 0105-2896
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- Citations: 12
Jamil KM, Alexander GJ, Cheent KS, et al., 2012, Hepatitis C virus infection abrogates the effect of liver transplantation on NK cell activation, 63rd Annual Meeting of the American-Association-for-the-Study-of-Liver-Diseases (AASLD), Publisher: WILEY-BLACKWELL, Pages: 1107A-1107A, ISSN: 0270-9139
Cheent KS, Jamil KM, Purbhoo M, et al., 2012, The HLA-G signal peptide antagonises NK cell inhibition due to HCV core35-44 at HLA-C, 63rd Annual Meeting of the American-Association-for-the-Study-of-Liver-Diseases (AASLD), Publisher: WILEY-BLACKWELL, Pages: 1103A-1103A, ISSN: 0270-9139
Purbhoo M, Kumar N, Ashraf S, 2012, LLT1 engages CD161 at the immunological synapse between T cells and hepatocytes, European Congress of Immunology, Publisher: WILEY-BLACKWELL, Pages: 504-504, ISSN: 0019-2805
Aletrari M, Sabaratnam K, Dyball S, et al., 2012, The role of inhibitory receptor affinity in differential partitioning of TCR at the immune synapse, European Congress of Immunology, Publisher: WILEY-BLACKWELL, Pages: 155-156, ISSN: 0019-2805
Purbhoo MA, Kumar N, Ashraf S, 2012, LLT1 ENGAGES CD161 AT THE IMMUNOLOGICAL SYNAPSE BETWEEN T CELLS AND HEPATOCYTES, 47th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL), Publisher: ELSEVIER SCIENCE BV, Pages: S315-S315, ISSN: 0168-8278
Sabaratnam K, Dyball S, Seyedmohammad S, et al., 2011, Viral ligands reposition activating and inhibitory receptors at the T-cell immune synapse to enhance inhibitory signalling: a novel mechanism for immune escape, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 48-48, ISSN: 0019-2805
Purbhoo MA, Liu H, Oddos S, et al., 2010, Dynamics of sub-synaptic vesicles and surface microclusters at the T cell immunological synapse, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 175-175, ISSN: 0019-2805
Borhis G, Purbhoo M, Ahmed PS, et al., 2010, Effects of MHC class I peptides on KIR2DL3 clustering and signalling, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 134-134, ISSN: 0019-2805
Liu H, Purbhoo MA, Davis DM, et al., 2010, SH2 domain containing leukocyte phosphoprotein of 76-kDa (SLP-76) feedback regulation of ZAP-70 microclustering, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 10166-10171, ISSN: 0027-8424
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- Citations: 14
Fadda L, Borhis G, Ahmed P, et al., 2010, Peptide antagonism as a mechanism for NK cell activation, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 10160-10165, ISSN: 0027-8424
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- Citations: 123
Purbhoo MA, Liu H, Oddos S, et al., 2010, Dynamics of Subsynaptic Vesicles and Surface Microclusters at the Immunological Synapse, SCIENCE SIGNALING, Vol: 3, ISSN: 1945-0877
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- Citations: 101
Varela-Rohena A, Molloy PE, Dunn SM, et al., 2008, Control of HIV-1 immune escape by CD8 T cells expressing enhanced T-cell receptor, NATURE MEDICINE, Vol: 14, Pages: 1390-1395, ISSN: 1078-8956
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- Citations: 199
Oddos S, Dunsby C, Purbhoo MA, et al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495
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- Citations: 57
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