Imperial College London
Alternate

ProfessorMarkJohnson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Clinical Chair in Obstetrics
 
 
 
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Contact

 

+44 (0)20 3315 7887mark.johnson

 
 
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Location

 

H3.35Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ibeto:2020:10.1371/journal.pone.0228507,
author = {Ibeto, L and Antonopoulos, A and Grassi, P and Pang, P-C and Panico, M and Bobdiwala, S and Al-Memar, M and Davis, P and Davis, M and Norman, Taylor J and Almeida, P and Johnson, MR and Harvey, R and Bourne, T and Seckl, M and Clark, G and Haslam, SM and Dell, A},
doi = {10.1371/journal.pone.0228507},
journal = {PLoS One},
title = {Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis},
url = {http://dx.doi.org/10.1371/journal.pone.0228507},
volume = {15},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy. Glycosylation of hCG is known to be essential for its biological activity. "Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases. However, what constitutes "hyperglycosylation" is not yet fully understood. In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG. hCG was isolated in urine collected from women at 7 weeks and 20 weeks' gestation. hCG was also isolated in urine from women diagnosed with gestational trophoblastic disease (GTD). We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples. The structures identified on the early pregnancy (EP-hCG) and late pregnancy (LP-hCG) samples corresponded to mono-, bi-, tri-, and tetra-antennary N-glycans. A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples. A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans. GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy. When compared to EP-hCG, GTD-hCG samples had decreased signals for tri- and tetra-antennary N-glycans. In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance. hCG carries the same N-glycans throughout pregnancy but in different propo
AU  - Ibeto,L
AU  - Antonopoulos,A
AU  - Grassi,P
AU  - Pang,P-C
AU  - Panico,M
AU  - Bobdiwala,S
AU  - Al-Memar,M
AU  - Davis,P
AU  - Davis,M
AU  - Norman,Taylor J
AU  - Almeida,P
AU  - Johnson,MR
AU  - Harvey,R
AU  - Bourne,T
AU  - Seckl,M
AU  - Clark,G
AU  - Haslam,SM
AU  - Dell,A
DO  - 10.1371/journal.pone.0228507
PY  - 2020///
SN  - 1932-6203
TI  - Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0228507
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32045434
UR  - http://hdl.handle.net/10044/1/77020
VL  - 15
ER  -
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