Publications
300 results found
Robinson T, Valluri P, Manning HB, et al., 2008, Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 1887-1889, ISSN: 0146-9592
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- Citations: 19
Poher V, Kennedy GT, Manning HB, et al., 2008, Improved sectioning in a slit scanning confocal microscope, OPTICS LETTERS, Vol: 33, Pages: 1813-1815, ISSN: 0146-9592
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- Citations: 17
Galletly NP, McGinty J, Dunsby C, et al., 2008, Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin, BRITISH JOURNAL OF DERMATOLOGY, Vol: 159, Pages: 152-161, ISSN: 0007-0963
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- Citations: 104
Poher V, Grossman N, Kennedy GT, et al., 2008, Micro-LED arrays: a tool for two-dimensional neuron stimulation, Journal of Physics D: Applied Physics, Vol: 41, Pages: 094014-094023, ISSN: 0022-3727
Gong Z, Gu E, Jin SR, et al., 2008, Efficient flip-chip InGaN micro-pixellated light-emitting diode arrays: Promising candidates for micro-displays and colour conversion, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 41, ISSN: 0022-3727
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- Citations: 44
Dawson MD, Neil MAA, 2008, Micro-pixellated LEDs for science and instrumentation, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 41, ISSN: 0022-3727
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- Citations: 12
Kennedy GT, Elson DS, Hares JD, et al., 2008, Fluorescence lifetime imaging using light emitting diodes, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 41, ISSN: 0022-3727
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- Citations: 5
Auksorius E, Boruah BR, Dunsby C, et al., 2008, Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 113-115, ISSN: 0146-9592
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- Citations: 143
McGinty J, Tahir KB, Soloviev VY, et al., 2008, Time-domain fluorescence lifetime tomography
We present a platform for fluorescence lifetime tomography utilising tuneable supercontinuum excitation and wide-field time-gated technology. Applied to optical projection and diffuse fluorescence tomography, we demonstrate 3D time-resolved fluorescence reconstruction in transparent and scattering phantoms.
Nadeau VJ, Elson DS, Neil MAA, et al., 2008, Laser-Pumped Endoscopic Illumination Source, 30th Annual International Conference of the IEEE-Engineering-in-Medicine-and-Biology-Society, Publisher: IEEE, Pages: 2059-+, ISSN: 1557-170X
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- Citations: 7
Nadeau VJ, Elson DS, Neil MAA, et al., 2008, Laser-pumped endoscopic illumination source., Annu Int Conf IEEE Eng Med Biol Soc, Vol: 2008, Pages: 2059-2062, ISSN: 2375-7477
Light sources currently employed in endoscopy have a number of disadvantages including inefficiency, high temperature, non-uniform illumination, and the production of shadow-less images. In this paper, we present a novel endoscopic illumination source, based on the use of a blue-violet laser diode in combination with a yellow phosphor for the production of white light. By using this approach, the resulting illumination source is more compact and potentially more ergonomic than those currently used in endoscopy. Spectral measurements of the emitted light indicate that it has a colour similar to that of daylight, and images obtained under illumination with this light source indicate that it provides more uniform illumination and sharper shadows than standard endoscopic light sources.
Owen DM, Auksorius E, Manning HB, et al., 2007, Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source, OPTICS LETTERS, Vol: 32, Pages: 3408-3410, ISSN: 0146-9592
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- Citations: 54
Manning HB, Owen DM, Auksorius E, et al., 2007, Applications of rapid time-gated hyperspectral FLIM: Live cell imaging of membrane order and 6-D microscopy, ISSN: 1605-7422
We describe the characterisation of a hyperspectral fluorescence lifetime imaging microscope that exploits high-speed time-gated imaging technology and a tunable continuum source for 6-D fluorescence imaging. This line-scanning confocal microscope can record the full spectral-temporal (i.e. excitation-emission-lifetime) fluorescence matrix at each pixel in a three dimensional (x-y-z) sample. This instrument has been applied to biological samples including model membranes and live cells labelled with the phase-sensitive membrane dye di-4-ANEPPDHQ, for which significant variation of lifetime with emission wavelength is observed. © 2007 SPIE-OSA.
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, Optics Express, Vol: 15, Pages: 16656-16673
We present a time domain optically sectioned fluorescence lifetime imaging (FLIM) microscope developed for high-speed live cell imaging. This single photon excited system combines wide field parallel pixel detection with confocal sectioning utilizing spinning Nipkow disc microscopy. It can acquire fluorescence lifetime images of live cells at up to 10 frames per second (fps), permitting high-speed FLIM of cell dynamics and protein interactions with potential for high throughput cell imaging and screening applications. We demonstrate the application of this FLIM microscope to real-time monitoring of changes in lipid order in cell membranes following cholesterol depletion using cyclodextrin and to the activation of the small GTP-ase Ras in live cells using FRET. © 2007 Optical Society of America.
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, OPTICS EXPRESS, Vol: 15, Pages: 15656-15673, ISSN: 1094-4087
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- Citations: 65
Benninger RK, Hofmann O, Onfelt B, et al., 2007, Fluorescence-Lifetime Imaging of DNA-Dye Interactions within Continuous-Flow Microfluidic Systems., Angew Chem Int Ed Engl, Vol: 46, Pages: 8536-8536, ISSN: 1433-7851
Soloviev VY, Tahir KB, McGinty J, et al., 2007, Fluorescence lifetime imaging by using time-gated data acquisition, APPLIED OPTICS, Vol: 46, Pages: 7384-7391, ISSN: 1559-128X
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- Citations: 49
Kumar S, Dunsby C, De Beule PAA, et al., 2007, Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging, Optics Express, Vol: 15, Pages: 12548-12561
We report a multifocal multiphoton time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode PMT detector. Multiphoton excitation minimizes out-of-focus photobleaching, multifocal excitation reduces non-linear in-plane photobleaching effects and TCSPC electronics provide photon-efficient detection of the fluorescence decay profile. TCSPC detection is less prone to bleaching- and movementinduced artefacts compared to wide-field time-gated or frequency-domain FLIM. This microscope is therefore capable of acquiring 3-D FLIM images at significantly increased speeds compared to single beam multiphoton microscopy and we demonstrate this with live cells expressing a GFP tagged protein. We also apply this system to time-lapse FLIM of NAD(P)H autofluorescence in single live cells and report measurements on the change in the fluorescence decay profile following the application of a known metabolic inhibitor.
Gong Z, Zhang HX, Gu E, et al., 2007, Matrix-addressable micropixellated InGaN light-emitting diodes with uniform emission and increased light output, IEEE TRANSACTIONS ON ELECTRON DEVICES, Vol: 54, Pages: 2650-2658, ISSN: 0018-9383
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- Citations: 53
Owen DM, Neil MAA, French PMW, et al., 2007, Optical techniques for imaging membrane lipid microdomains in living cells, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, Vol: 18, Pages: 591-598, ISSN: 1084-9521
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- Citations: 34
Poher V, Zhang HX, Kennedy GT, et al., 2007, Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode, OPTICS EXPRESS, Vol: 15, Pages: 11196-11206, ISSN: 1094-4087
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- Citations: 45
Garcia DI, Lanigan P, Webb M, et al., 2007, Fluorescence lifetime imaging to detect actomyosin states in mammalian muscle sarcomeres, BIOPHYSICAL JOURNAL, Vol: 93, Pages: 2091-2101, ISSN: 0006-3495
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- Citations: 9
Soloviev VY, McGinty J, Tahir KB, et al., 2007, Fluorescence lifetime tomography of live cells expressing enhanced green fluorescent protein embedded in a scattering medium exhibiting background autofluorescence, OPTICS LETTERS, Vol: 32, Pages: 2034-2036, ISSN: 0146-9592
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- Citations: 23
Soloviev VY, Tahir KB, McGinty J, et al., 2007, Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data, European Conference on Biomedical Optics, Publisher: SPIE
De Beule P, Owen DM, Manning HB, et al., 2007, Rapid hyperspectral fluorescence lifetime imaging, MICROSCOPY RESEARCH AND TECHNIQUE, Vol: 70, Pages: 481-484, ISSN: 1059-910X
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- Citations: 44
Hegyi L, Talbot C, Monaco C, et al., 2007, Fluorescence lifetime imaging of unstained human atherosclerotic plaques., The 14th International Symposium on Atherosclerosis
Boruah BR, Neil MAA, 2007, Programmable diffractive optics for laser scanning confocal microscopy, Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XIV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 3
Kennedy GT, Elson DS, Munro I, et al., 2007, Fluorescence lifetime imaging using light emitting diodes, Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XIV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
Benninger RKP, Hofmann O, Onfelt B, et al., 2007, Fluorescence-lifetime imaging of DNA-Dye interactions within continuous-flow microfluidic systems (vol 46, pg 8525, 2007), ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 46, Pages: 8536-8536, ISSN: 1433-7851
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- Citations: 4
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