Imperial College London
Alternate

ProfessorMarkNeil

Faculty of Natural Sciences, Department of Physics

Professor of Photonics
 
 
 
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Contact

 

+44 (0)20 7594 7611mark.neil

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

608Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Quicke:2019:10.3389/fncel.2019.00202,
author = {Quicke, P and Song, C and McKimm, EJ and Milosevic, MM and Howe, CL and Neil, M and Schultz, SR and Antic, SD and Foust, AJ and Knopfel, T},
doi = {10.3389/fncel.2019.00202},
journal = {Frontiers in Cellular Neuroscience},
title = {Corrigendum: Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators},
url = {http://dx.doi.org/10.3389/fncel.2019.00202},
volume = {13},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Voltage imaging of many neurons simultaneously at single-cell resolution is hampered bythe difficulty of detecting small voltage signals from overlapping neuronal processes inneural tissue. Recent advances in genetically encoded voltage indicator (GEVI) imaginghave shown single-cell resolution optical voltage recordings in intact tissue throughimaging naturally sparse cell classes, sparse viral expression, soma restricted expression,advanced optical systems, or a combination of these. Widespread sparse and strongtransgenic GEVI expression would enable straightforward optical access to a denselyoccurring cell type, such as cortical pyramidal cells. Here we demonstrate that a recentlydescribed sparse transgenic expression strategy can enable single-cell resolution voltageimaging of cortical pyramidal cells in intact brain tissue without restricting expression tothe soma. We also quantify the functional crosstalk in brain tissue and discuss optimalimaging rates to inform future GEVI experimental design.
AU  - Quicke,P
AU  - Song,C
AU  - McKimm,EJ
AU  - Milosevic,MM
AU  - Howe,CL
AU  - Neil,M
AU  - Schultz,SR
AU  - Antic,SD
AU  - Foust,AJ
AU  - Knopfel,T
DO  - 10.3389/fncel.2019.00202
PY  - 2019///
SN  - 1662-5102
TI  - Corrigendum: Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators
T2  - Frontiers in Cellular Neuroscience
UR  - http://dx.doi.org/10.3389/fncel.2019.00202
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000467478300002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/70368
VL  - 13
ER  -
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