Imperial College London
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Professor Matthew Fisher

Faculty of Medicine, School of Public Health

Professor of Fungal Disease Epidemiology
 
 
 
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Contact

 

matthew.fisher Website

 
 
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Location

 

1113Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Ghosh:2020:10.1101/2020.08.14.250787,
author = {Ghosh, PN and Verster, R and Sewell, T and OHanlon, S and Brookes, LM and Rieux, A and Garner, TWJ and Weldon, C and Fisher, MC},
doi = {10.1101/2020.08.14.250787},
title = {Discriminating Lineages of <i>Batrachochytrium dendrobatidis</i> using quantitative PCR},
url = {http://dx.doi.org/10.1101/2020.08.14.250787},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>The ability to detect and monitor infectious disease in a phylogenetically informative manner is critical for their management. Phylogenetically informative diagnostic tests enable patterns of pathogen introduction or changes in the distribution of genotypes to be measured, enabling research into the ecology of the pathogen. <jats:italic>Batrachochytrium dendrobatidis</jats:italic> (<jats:italic>Bd</jats:italic>), a causative agent of chytridiomycosis in amphibian populations, emerged worldwide in the 21<jats:sup>st</jats:sup> century and is composed of six lineages which are display varying levels of virulence in their hosts.</jats:p><jats:p>Research into the distribution, ecology and pathogenicity of these lineages has been hampered by an inability to type lineage efficiently. Here, we describe a lineage-specific TaqMan qPCR assay that differentiates the two lineages of <jats:italic>Bd</jats:italic> most commonly associated with chytridiomycosis: <jats:italic>Bd</jats:italic>GPL and <jats:italic>Bd</jats:italic>CAPE. We demonstrate how this assay can be used for the surveillance of wild populations of amphibians in Southern Africa using skin swabs, tissue samples and cultured isolates.</jats:p>
AU  - Ghosh,PN
AU  - Verster,R
AU  - Sewell,T
AU  - OHanlon,S
AU  - Brookes,LM
AU  - Rieux,A
AU  - Garner,TWJ
AU  - Weldon,C
AU  - Fisher,MC
DO  - 10.1101/2020.08.14.250787
PY  - 2020///
TI  - Discriminating Lineages of <i>Batrachochytrium dendrobatidis</i> using quantitative PCR
UR  - http://dx.doi.org/10.1101/2020.08.14.250787
ER  -
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