Imperial College London
Portrait Picture

Professor Matthew Fisher

Faculty of Medicine, School of Public Health

Professor of Fungal Disease Epidemiology
 
 
 
//

Contact

 

matthew.fisher Website

 
 
//

Location

 

1113Sir Michael Uren HubWhite City Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Furukawa:2022:10.1101/2022.03.28.486018,
author = {Furukawa, T and van, Rhijn N and Chown, H and Rhodes, J and Alfuraji, N and Fortune-Grant, R and Bignell, E and Fisher, MC and Bromley, M},
doi = {10.1101/2022.03.28.486018},
title = {Exploring a novel genomic safe-haven site in the human pathogenic mould <i>Aspergillus fumigatus</i>},
url = {http://dx.doi.org/10.1101/2022.03.28.486018},
year = {2022}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>ABSTRACT</jats:title><jats:p><jats:italic>Aspergillus fumigatus</jats:italic> is the most important airborne fungal pathogen and allergen of humans causing high morbidity and mortality worldwide. The factors that govern pathogenicity of this organism are multi-factorial and are poorly understood. Molecular tools to dissect the mechanisms of pathogenicity in <jats:italic>A. fumigatus</jats:italic> have improved significantly over the last 20 years however many procedures have not been standardised for <jats:italic>A. fumigatus</jats:italic>. Here, we present a new genomic safe-haven locus at the site of an inactivated transposon, named SH-<jats:italic>aft4</jats:italic>, which can be used to insert DNA sequences in the genome of this fungus without impacting its phenotype. We show that we are able to effectively express a transgene construct from the SH-<jats:italic>aft4</jats:italic> and that natural regulation of promoter function is conserved at this site. Furthermore, the SH-<jats:italic>aft4</jats:italic> locus is highly conserved in the genome of a wide range of clinical and environmental isolates including the isolates commonly used by many laboratories CEA10, Af293 and ATCC46645, allowing a wide range of isolates to be manipulated. Our results show that the <jats:italic>aft4</jats:italic> locus can serve as a site for integration of a wide range of genetic constructs to aid functional genomics studies of this important human fungal pathogen.</jats:p>
AU  - Furukawa,T
AU  - van,Rhijn N
AU  - Chown,H
AU  - Rhodes,J
AU  - Alfuraji,N
AU  - Fortune-Grant,R
AU  - Bignell,E
AU  - Fisher,MC
AU  - Bromley,M
DO  - 10.1101/2022.03.28.486018
PY  - 2022///
TI  - Exploring a novel genomic safe-haven site in the human pathogenic mould <i>Aspergillus fumigatus</i>
UR  - http://dx.doi.org/10.1101/2022.03.28.486018
ER  -
Download