Imperial College London
Alternate

ProfessorMatthewPickering

Faculty of Medicine, Department of Immunology and Inflammation

Centre Director, Professor of Rheumatology
 
 
 
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Contact

 

matthew.pickering Website

 
 
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Assistant

 

Miss Claudia Rocchi +44 (0)20 3313 2315

 
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Location

 

9N12Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gilmore:2022:10.1136/lupus-2021-000615,
author = {Gilmore, A and Wilson, H and Cairns, T and Botto, M and Lightstone, L and Bruce, I and Cook, H and Pickering, M and on, behalf of the MASTERPLANS Consortium},
doi = {10.1136/lupus-2021-000615},
journal = {Lupus Science & Medicine},
title = {Immune gene expression and functional networks in distinct lupus nephritis classes},
url = {http://dx.doi.org/10.1136/lupus-2021-000615},
volume = {9},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Objective: To explore the utility of the NanoString platform in elucidating kidney immune transcripts for class III, IV and V lupus nephritis (LN) using a retrospective cohort of formalin-fixed paraffin-embedded (FFPE) kidney biopsy tissue.Methods: Immune gene transcript analysis was performed using the NanoString nCounter platform on RNA from LN (n=55), thin basement membrane disease (TBM, n=14) and membranous nephropathy (MN, n=9) FFPE kidney biopsy tissue. LN samples consisted of single class III (n=11), IV (n=23) and V (n=21) biopsies with no mixed lesions. Differential gene expression was performed with NanoString nSolver, with visualisations of volcano plots and heatmaps generated in R. Significant transcripts were interrogated to identify functional networks using STRING and Gene ontogeny terms.  Results: In comparison to TBM, we identified 52 significantly differentially expressed genes common to all three LN classes. Pathway analysis showed enrichment for type I interferon (IFN) signalling, complement and MHC II pathways, with most showing the highest expression in class IV LN. Our class IV LN biopsies also showed significant upregulation of NF-κB signalling and immunological enrichment in comparison to class V LN biopsies. Transcripts from the type I IFN pathway distinguished class V LN from MN.  Conclusion: Our whole kidney section transcriptomic analysis provided insights into the molecular profile of class III, IV and V LN. The data highlighted important pathways common to all three classes and pathways enriched in our class IV LN biopsies. The ability to reveal molecular pathways in LN using FFPE whole biopsy sections could have clinical utility in treatment selection for LN.
AU  - Gilmore,A
AU  - Wilson,H
AU  - Cairns,T
AU  - Botto,M
AU  - Lightstone,L
AU  - Bruce,I
AU  - Cook,H
AU  - Pickering,M
AU  - on,behalf of the MASTERPLANS Consortium
DO  - 10.1136/lupus-2021-000615
PY  - 2022///
SN  - 2053-8790
TI  - Immune gene expression and functional networks in distinct lupus nephritis classes
T2  - Lupus Science & Medicine
UR  - http://dx.doi.org/10.1136/lupus-2021-000615
UR  - https://lupus.bmj.com/content/9/1/e000615
UR  - http://hdl.handle.net/10044/1/93833
VL  - 9
ER  -
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