Imperial College London
Alternate

ProfessorMatthiasMerkenschlager

Faculty of Medicine, Institute of Clinical Sciences

Professor of Cell Biology
 
 
 
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Contact

 

+44 (0)20 3313 8239matthias.merkenschlager

 
 
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Location

 

5.11DLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rojec:2019:10.7554/eLife.49038,
author = {Rojec, M and Hocher, A and Stevens, KM and Merkenschlager, M and Warnecke, T},
doi = {10.7554/eLife.49038},
journal = {eLife},
pages = {1--23},
title = {Chromatinization of Escherichia coli with archaeal histones},
url = {http://dx.doi.org/10.7554/eLife.49038},
volume = {8},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Nucleosomes restrict DNA accessibility throughout eukaryotic genomes, withrepercussions for replication, transcription, and other DNA-templated processes. How this globallyrestrictive organization emerged during evolution remains poorly understood. Here, to betterunderstand the challenges associated with establishing globally restrictive chromatin, we expresshistones in a naive system that has not evolved to deal with nucleosomal structures: Escherichiacoli. We find that histone proteins from the archaeon Methanothermus fervidus assemble on the E.coli chromosome in vivo and protect DNA from micrococcal nuclease digestion, allowing us to mapbinding footprints genome-wide. We show that higher nucleosome occupancy at promoters isassociated with lower transcript levels, consistent with local repressive effects. Surprisingly,however, this sudden enforced chromatinization has only mild repercussions for growth unless cellsexperience topological stress. Our results suggest that histones can become established asubiquitous chromatin proteins without interfering critically with key DNA-templated processes.
AU  - Rojec,M
AU  - Hocher,A
AU  - Stevens,KM
AU  - Merkenschlager,M
AU  - Warnecke,T
DO  - 10.7554/eLife.49038
EP  - 23
PY  - 2019///
SN  - 2050-084X
SP  - 1
TI  - Chromatinization of Escherichia coli with archaeal histones
T2  - eLife
UR  - http://dx.doi.org/10.7554/eLife.49038
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000498686000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://elifesciences.org/articles/49038
UR  - http://hdl.handle.net/10044/1/76963
VL  - 8
ER  -
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