Imperial College London
Alternate

ProfessorMatthiasMerkenschlager

Faculty of Medicine, Institute of Clinical Sciences

Professor of Cell Biology
 
 
 
//

Contact

 

+44 (0)20 3313 8239matthias.merkenschlager

 
 
//

Location

 

5.11DLMS BuildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Liang:2017:10.7554/eLife.22767,
author = {Liang, Z and Brown, KE and Carroll, T and Taylor, B and Vidal, IF and Hendrich, B and Rueda, D and Fisher, AG and Merkenschlager, M},
doi = {10.7554/eLife.22767},
journal = {ELIFE},
title = {A high-resolution map of transcriptional repression},
url = {http://dx.doi.org/10.7554/eLife.22767},
volume = {6},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Turning genes on and off is essential for development and homeostasis, yet little is known about the sequence and causal role of chromatin state changes during the repression of active genes. This is surprising, as defective gene silencing underlies developmental abnormalities and disease. Here we delineate the sequence and functional contribution of transcriptional repression mechanisms at high temporal resolution. Inducible entry of the NuRD-interacting transcriptional regulator Ikaros into mouse pre-B cell nuclei triggered immediate binding to target gene promoters. Rapid RNAP2 eviction, transcriptional shutdown, nucleosome invasion, and reduced transcriptional activator binding required chromatin remodeling by NuRD-associated Mi2beta/CHD4, but were independent of HDAC activity. Histone deacetylation occurred after transcriptional repression. Nevertheless, HDAC activity contributed to stable gene silencing. Hence, high resolution mapping of transcriptional repression reveals complex and interdependent mechanisms that underpin rapid transitions between transcriptional states, and elucidates the temporal order, functional role and mechanistic separation of NuRD-associated enzymatic activities.
AU  - Liang,Z
AU  - Brown,KE
AU  - Carroll,T
AU  - Taylor,B
AU  - Vidal,IF
AU  - Hendrich,B
AU  - Rueda,D
AU  - Fisher,AG
AU  - Merkenschlager,M
DO  - 10.7554/eLife.22767
PY  - 2017///
SN  - 2050-084X
TI  - A high-resolution map of transcriptional repression
T2  - ELIFE
UR  - http://dx.doi.org/10.7554/eLife.22767
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000397897600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/46061
VL  - 6
ER  -
Download