Imperial College London
Alternate

Dr Melissa Wickremasinghe

Faculty of Medicine, National Heart & Lung Institute

Honorary Clinical Senior Lecturer
 
 
 
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Contact

 

melissa.wickremasinghe

 
 
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Location

 

Praed StreetSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thillai:2012:10.1371/journal.pone.0038083,
author = {Thillai, M and Eberhardt, C and Lewin, AM and Potiphar, L and Hingley-Wilson, S and Sridhar, S and Macintyre, J and Kon, OM and Wickremasinghe, M and Wells, A and Weeks, ME and Mitchell, D and Lalvani, A},
doi = {10.1371/journal.pone.0038083},
journal = {PLoS One},
title = {Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood},
url = {http://dx.doi.org/10.1371/journal.pone.0038083},
volume = {7},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: The clinical, radiological and pathological similarities between sarcoidosis and tuberculosis can make disease differentiation challenging. A complicating factor is that some cases of sarcoidosis may be initiated by mycobacteria. We hypothesised that immunological profiling might provide insight into a possible relationship between the diseases or allow us to distinguish between them. METHODS: We analysed bronchoalveolar lavage (BAL) fluid in sarcoidosis (n = 18), tuberculosis (n = 12) and healthy volunteers (n = 16). We further investigated serum samples in the same groups; sarcoidosis (n = 40), tuberculosis (n = 15) and healthy volunteers (n = 40). A cross-sectional analysis of multiple cytokine profiles was performed and data used to discriminate between samples. RESULTS: We found that BAL profiles were indistinguishable between both diseases and significantly different from healthy volunteers. In sera, tuberculosis patients had significantly lower levels of the Th2 cytokine interleukin-4 (IL-4) than those with sarcoidosis (p = 0.004). Additional serum differences allowed us to create a linear regression model for disease differentiation (within-sample accuracy 91%, cross-validation accuracy 73%). CONCLUSIONS: These data warrant replication in independent cohorts to further develop and validate a serum cytokine signature that may be able to distinguish sarcoidosis from tuberculosis. Systemic Th2 cytokine differences between sarcoidosis and tuberculosis may also underly different disease outcomes to similar respiratory stimuli.
AU  - Thillai,M
AU  - Eberhardt,C
AU  - Lewin,AM
AU  - Potiphar,L
AU  - Hingley-Wilson,S
AU  - Sridhar,S
AU  - Macintyre,J
AU  - Kon,OM
AU  - Wickremasinghe,M
AU  - Wells,A
AU  - Weeks,ME
AU  - Mitchell,D
AU  - Lalvani,A
DO  - 10.1371/journal.pone.0038083
PY  - 2012///
SN  - 1932-6203
TI  - Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0038083
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22815689
UR  - http://hdl.handle.net/10044/1/43527
VL  - 7
ER  -
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