Imperial College London

DrMichaelCox

Faculty of MedicineNational Heart & Lung Institute

Research Associate
 
 
 
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Contact

 

+44 (0)20 7594 7974michael.cox1 Website

 
 
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Location

 

413Guy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Cox:2016:10.1101/050237,
author = {Cox, MJ and Turek, EM and Hennessy, C and Mirza, GK and James, PL and Coleman, M and Jones, A and Wilson, R and Bilton, D and Cookson, WOCM and Moffatt, MF and Loebinger, M},
doi = {10.1101/050237},
publisher = {Cold Spring Harbor Laboratory},
title = {Longitudinal assessment of sputum microbiome by sequencing of the 16S rRNA gene in non-CF bronchiectasis patients},
url = {http://dx.doi.org/10.1101/050237},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - UNPB
AB - <jats:p>Bronchiectasis is accompanied by chronic bronchial infection that may drive disease progression. However, the evidence base for antibiotic therapy is limited. DNA based methods offer better identification and quantification of microbial constituents of sputum than standard clinical culture and may help inform patient management strategies. Our study objective was to determine the longitudinal variability of the non-CF bronchiectasis microbiome in sputum with respect to clinical variables. Eighty-five patients with non-cystic fibrosis (CF) bronchiectasis and daily sputum production were recruited from outpatient clinics and followed for six months. Monthly sputum samples and clinical measurements were taken, together with additional samples during exacerbations. 16S rRNA gene sequencing of the sputum microbiota was successful for 381 samples from 76 patients and analysed in conjunction with clinical data. Microbial communities were highly individual in composition and stability, usually with limited diversity and often containing multiple pathogens. When compared to DNA sequencing, microbial culture had restricted sensitivity in identifying common pathogens. With some exceptions, community characteristics showed poor correlations with clinical features including underlying disease, antibiotic use and exacerbations. The use of microbial community analysis of sputum added to information from microbial culture. A simple model of exacerbations driven by bacterial overgrowth was not supported, suggesting a need for revision of principles for antibiotic therapy. In individual patients, the management of chronic bronchial infection may be improved by therapy specific to their microbiome, taking into account pathogen load, community stability, and acute and chronic community responses to antibiotics.</jats:p>
AU - Cox,MJ
AU - Turek,EM
AU - Hennessy,C
AU - Mirza,GK
AU - James,PL
AU - Coleman,M
AU - Jones,A
AU - Wilson,R
AU - Bilton,D
AU - Cookson,WOCM
AU - Moffatt,MF
AU - Loebinger,M
DO - 10.1101/050237
PB - Cold Spring Harbor Laboratory
PY - 2016///
TI - Longitudinal assessment of sputum microbiome by sequencing of the 16S rRNA gene in non-CF bronchiectasis patients
UR - http://dx.doi.org/10.1101/050237
ER -