Publications
222 results found
Spinardi L, Rietdorf J, Nitsch L, et al., 2004, A dynamic podosome-like structure of epithelial cells, EXPERIMENTAL CELL RESEARCH, Vol: 295, Pages: 360-374, ISSN: 0014-4827
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- Citations: 89
Gasman S, Chasserot-Golaz S, Malacombe M, et al., 2004, Regulated exocytosis in neuroendocrine cells: A role for subplasmalemmal Cdc42/N-WASP-induced actin filaments, MOLECULAR BIOLOGY OF THE CELL, Vol: 15, Pages: 520-531, ISSN: 1059-1524
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- Citations: 143
Zeng R, Cannon JL, Abraham RT, et al., 2003, SLP-76 coordinates Nck-dependent Wiskott-Aldrich syndrome protein recruitment with Vav-1/Cdc42-dependent Wiskott-Aldrich syndrome protein activation at the T cell-APC contact site, JOURNAL OF IMMUNOLOGY, Vol: 171, Pages: 1360-1368, ISSN: 0022-1767
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- Citations: 130
Grosse R, Copeland JW, Newsome TP, et al., 2003, A role for VASP in RhoA-diaphanous signalling to actin dynamics and SRF activity, EMBO JOURNAL, Vol: 22, Pages: 3050-3061, ISSN: 0261-4189
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- Citations: 81
Garvalov BK, Higgins TE, Sutherland JD, et al., 2003, The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions, JOURNAL OF CELL BIOLOGY, Vol: 161, Pages: 33-39, ISSN: 0021-9525
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- Citations: 61
Harlander RS, Way M, Ren Q, et al., 2003, Effects of ectopically expressed neuronal Wiskott-Aldrich syndrome protein domains on <i>Rickettsia rickettsii</i> actin-based motility, INFECTION AND IMMUNITY, Vol: 71, Pages: 1551-1556, ISSN: 0019-9567
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- Citations: 28
Benesch S, Lommel S, Steffen A, et al., 2002, Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 277, Pages: 37771-37776, ISSN: 0021-9258
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- Citations: 123
Zettl M, Way M, 2002, The WH1 and EVH1 domains of WASP and Ena/VASP family members bind distinct sequence motifs, CURRENT BIOLOGY, Vol: 12, Pages: 1617-1622, ISSN: 0960-9822
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- Citations: 57
Sutherland JD, Way M, 2002, Looking over the edge: A new role for Ena/VASP proteins in lamellipodial dynamics, DEVELOPMENTAL CELL, Vol: 2, Pages: 692-694, ISSN: 1534-5807
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- Citations: 4
Scaplehorn N, Holmström A, Moreau V, et al., 2002, Grb2 and Nck act cooperatively to promote actin-based motility of vaccinia virus, CURRENT BIOLOGY, Vol: 12, Pages: 740-745, ISSN: 0960-9822
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- Citations: 118
Luna A, Matas OB, Martínez-Menárguez JA, et al., 2002, Regulation of protein transport from the golgi complex to the endoplasmic reticulum by CDC42 and N-WASP, MOLECULAR BIOLOGY OF THE CELL, Vol: 13, Pages: 866-879, ISSN: 1059-1524
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- Citations: 124
Jiang ZY, Chawla A, Bose A, et al., 2002, A phosphatidylinositol 3-kinase-independent insulin signaling pathway to N-WASP/Arp2/3/F-actin required for GLUT4 glucose transporter recycling, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 277, Pages: 509-515, ISSN: 0021-9258
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- Citations: 122
McGee K, Zettl M, Way M, et al., 2001, A role for N-WASP in invasin-promoted internalisation, FEBS LETTERS, Vol: 509, Pages: 59-65, ISSN: 0014-5793
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- Citations: 46
Rietdorf J, Ploubidou A, Reckmann I, et al., 2001, Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus, NATURE CELL BIOLOGY, Vol: 3, Pages: 992-1000, ISSN: 1465-7392
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- Citations: 224
Ploubidou A, Rietdorf J, Reckmann I, et al., 2001, Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus at the plasma membrane, MOLECULAR BIOLOGY OF THE CELL, Vol: 12, Pages: 138A-138A, ISSN: 1059-1524
Zettl M, Way M, 2001, New tricks for an old dog?, NATURE CELL BIOLOGY, Vol: 3, Pages: E74-E75, ISSN: 1465-7392
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- Citations: 18
Ploubidou A, Way M, 2001, Viral transport and the cytoskeleton, CURRENT OPINION IN CELL BIOLOGY, Vol: 13, Pages: 97-105, ISSN: 0955-0674
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- Citations: 107
Frishknecht F, Way M, 2001, Surfing pathogens and the lessons learned for actin polymerization, TRENDS IN CELL BIOLOGY, Vol: 11, Pages: 30-38, ISSN: 0962-8924
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- Citations: 171
Defacque H, Egeberg M, Antzberger A, et al., 2000, Actin assembly induced by polylysine beads or purified phagosomes: Quantitation by a new flow cytometry assay, Cytometry, Vol: 41, Pages: 46-54, ISSN: 0196-4763
Background: Actin assembly on biological membranes is a poorly understood process. We have previously shown that phagosomal membranes could induce actin assembly in the presence of thymosin β4 (an actin sequestering protein that inhibits nonspecific nucleation), via the barbed ends of actin filaments. Methods: Here, we have developed an in vitro system based on fluorescein-labeled G (monomeric) actin and flow cytometry analysis, which allowed us to quantify de novo actin assembly on the cytoplasmic side of purified phagosomes. To standardize the system, we also used latex beads covalently coupled with polylysine, which efficiently promote actin nucleation. Results: Flow cytometry analysis showed that the percentage of polylysine beads positive for F-actin filaments increased in a time- and G-actin concentration-dependent manner. Incubation of phagosomes with reagents affecting actin dynamics allowed us to extend our previous data showing that the phagosomal membranes assemble actin filaments de novo. Finally, our results pin-point a potential role for gelsolin as a positive regulator of actin assembly on the phagosomal membrane. Conclusions: We propose that our system could facilitate the development of other in vitro assays for the analysis of actin assembly and its links to signaling in cells. (C) 2000 Wiley-Liss, Inc.
Defacque H, Egeberg M, Antzberger A, et al., 2000, Actin assembly induced by polylysine beads or purified phagosomes: Quantitation by a new flow cytometry assay, CYTOMETRY, Vol: 41, Pages: 46-54, ISSN: 0196-4763
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- Citations: 16
Ploubidou A, Moreau V, Ashman K, et al., 2000, Vaccinia virus infection disrupts microtubule organization and centrosome function, EMBO Workshop on Functional Organization of the Cell Nucleus, Publisher: WILEY, Pages: 3932-3944, ISSN: 0261-4189
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- Citations: 133
Moreau V, Frischknecht F, Reckmann I, et al., 2000, A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization, NATURE CELL BIOLOGY, Vol: 2, Pages: 441-448, ISSN: 1465-7392
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- Citations: 267
Ngai SM, Way MMY, Chan H, et al., 2000, <i>In silico</i> studies of energy metabolism of normal and diseased heart, MOLECULAR BIOLOGY REPORTS, Vol: 27, Pages: 123-128, ISSN: 0301-4851
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- Citations: 2
Huber LA, Fialka I, Paiha K, et al., 2000, Both calmodulin and the unconventional myosin Myr4 regulate membrane trafficking along the recycling pathway of MDCK cells, TRAFFIC, Vol: 1, Pages: 494-503, ISSN: 1398-9219
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- Citations: 65
Frischknecht F, Moreau V, Röttger S, et al., 1999, Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling, NATURE, Vol: 401, Pages: 926-929, ISSN: 0028-0836
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- Citations: 338
Gonfloni S, Frischknecht F, Way M, et al., 1999, Leucine 255 of Src couples intramolecular interactions to inhibition of catalysis, NATURE STRUCTURAL BIOLOGY, Vol: 6, Pages: 760-764, ISSN: 1072-8368
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- Citations: 61
Luetterforst R, Stang E, Zorzi N, et al., 1999, Molecular characterization of caveolin association with the Golgi complex: Identification of a cis-Golgi targeting domain in the caveolin molecule, JOURNAL OF CELL BIOLOGY, Vol: 145, Pages: 1443-1459, ISSN: 0021-9525
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- Citations: 107
Röttger S, Frischknecht F, Reckmann I, et al., 1999, Interactions between vaccinia virus IEV membrane proteins and their roles in IEV assembly and actin tail formation, JOURNAL OF VIROLOGY, Vol: 73, Pages: 2863-2875, ISSN: 0022-538X
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- Citations: 112
Salmon ED, Way M, 1999, Cytoskeleton, CURRENT OPINION IN CELL BIOLOGY, Vol: 11, Pages: 15-17, ISSN: 0955-0674
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- Citations: 10
Moreau V, Way M, 1999, <i>In vitro</i> approaches to study actin and microtubule dependent cell processes, CURRENT OPINION IN CELL BIOLOGY, Vol: 11, Pages: 152-158, ISSN: 0955-0674
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- Citations: 17
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