Imperial College London
Dr Michael Wilson

DrMichaelWilson

Faculty of Medicine, Department of Surgery & Cancer

Reader in Applied Respiratory Physiology
 
 
 
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Contact

 

+44 (0)20 3315 8292michael.wilson

 
 
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Location

 

G.3.43Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Soni:2020:10.1183/13993003.congress-2020.4726,
author = {Soni, S and Romano, R and O'Dea, K and Patel, B and Tatham, K and Koh, M and Tsiridou, D and Simon, A and Thakuria, L and Wilson, M and Reed, A and Marczin, N and Takata, M},
doi = {10.1183/13993003.congress-2020.4726},
pages = {1--2},
publisher = {European Respiratory Society},
title = {Intra-alveolar neutrophil-derived microvesicles predict development of primary graft dysfunction after lung transplantation},
url = {http://dx.doi.org/10.1183/13993003.congress-2020.4726},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - CPAPER
AB  - Background: Microvesicles (MV) play important roles in mediating intra-alveolar inflammation during acute lung injury. We investigated the MV profiles within bronchoalveolar lavage fluid (BALF) in patients undergoing lung transplantation and assessed relationship to the development of primary graft dysfunction (PGD).Methods: Lung transplant patients underwent bronchoscopy after completion of surgery and BALF samples (n=31) were analysed by flow cytometry for MVs derived from leucocytes (CD45+), neutrophils (CD66b+/CD11b+), monocytes (CD45+/CD14+), alveolar macrophages (CD206+/CD71+), platelets (CD31+/42b+) and epithelial (EpCAM+/T1α+) cells. MV numbers were correlated to clinical outcomes including hypoxia and development of PGD.Results: Various MV subpopulations were identified in BALF. Neutrophil-derived MVs were the largest population (10,548 (3,925-51,529)) compared to leucocyte MVs (9797 (4763-53444)), monocyte MVs (438 (196-25,627)), alveolar macrophage MVs (755 (343-6054) and platelets MVs (564 (286-953)) (MVs/µL, median (IQR)). Patients with PaO2/FiO2 (P/F) ratio ≤ 200mmHg at 72 hours had significantly higher levels of BALF neutrophil MVs than those with P/F ratio > 200mmHg (p=0.001). Patients who developed PGD also had significantly higher BALF neutrophil MVs than those who did not (p=0.03).Conclusions: We have undertaken a comprehensive evaluation of MVs within BALF of lung transplant recipients and demonstrated an association of BALF neutrophil MV numbers and PGD. Our data may suggest BALF neutrophil MVs as a potential, clinically relevant biomarker for PGD. Moreover, these MV may also have a pathogenic/inflammatory role in development of PGD
AU  - Soni,S
AU  - Romano,R
AU  - O'Dea,K
AU  - Patel,B
AU  - Tatham,K
AU  - Koh,M
AU  - Tsiridou,D
AU  - Simon,A
AU  - Thakuria,L
AU  - Wilson,M
AU  - Reed,A
AU  - Marczin,N
AU  - Takata,M
DO  - 10.1183/13993003.congress-2020.4726
EP  - 2
PB  - European Respiratory Society
PY  - 2020///
SN  - 0903-1936
SP  - 1
TI  - Intra-alveolar neutrophil-derived microvesicles predict development of primary graft dysfunction after lung transplantation
UR  - http://dx.doi.org/10.1183/13993003.congress-2020.4726
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000606501408008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://erj.ersjournals.com/content/56/suppl_64/4726
UR  - http://hdl.handle.net/10044/1/94042
ER  -
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